Biologia plantarum 52:783-786, 2008 | DOI: 10.1007/s10535-008-0153-x

Plant regeneration from callus culture of Curcuma aromatica and in vitro detection of somaclonal variation through cytophotometric analysis

S. Mohanty1, M. K. Panda1, E. Subudhi1, S. Nayak1,*
1 Centre of Biotechnology, Siksha 'O' Anusandhan University, Bhubaneswar, Orissa, India

Callus cultures initiated from shoot base explants of Curcuma aromatica Salisb. were maintained on Murashige and Skoog (MS) media supplemented with 2 mg dm-3 2,4-dichlorophenoxyacetic acid alone or with 0.5 mg dm-3 kinetin. Plantlets were regenerated from 60 and 180-d-old callus on MS media supplemented with 3 mg dm-3 benzyladenine and 0.5 mg dm-3 α-naphthalene acetic acid. Approximately 8-10 plantlets were produced after 30-40 d of culture per 50 mg of callus inoculated. Out of 113 regenerants analyzed 85 plants were exclusively diploid and 28 were predominantly diploid revealing presence of polyploid nuclei. Frequency of polyploid cells were more in regenerants obtained from 180-d-old callus then from 6-d-old callus which might be attributed to the ageing of callus.

Keywords: DNA content; tissue culture; wild turmeric
Subjects: auxins; Curcuma aromatica; cytokinins; in vitro culture, callus; in vitro culture, cell suspension; in vitro culture, growth control; in vitro culture, regeneration; nutrient medium, Murashige and Skoog (MS); ploidy

Received: November 17, 2007; Accepted: May 10, 2008; Published: December 1, 2008Show citation

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Mohanty, S., Panda, M.K., Subudhi, E., & Nayak, S. (2008). Plant regeneration from callus culture of Curcuma aromatica and in vitro detection of somaclonal variation through cytophotometric analysis. Biologia plantarum, 52(4), 783-786. doi: 10.1007/s10535-008-0153-x.
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