Biologia plantarum 44:19-24, 2001 | DOI: 10.1023/A:1017905917971

Effect of Some Antibiotics on the In Vitro Morphogenetic Response from Callus Cultures of Coryphantha Elephantidens

B.S. Bhau1, A.K. Wakhlu1
1 Plant Tissue Culture Laboratory, Department of Botany, University of Jammu, Jammu -, Jammu & Kashmir, India

The effect of five antibiotics: carbenicillin, chloramphenicol, cefotaxime, kanamycin and hygromycin on the organogenesis from callus cultures of Coryphantha elphantidens (Lem.) Lem. have been studied. Carbenicillin and cefotaxime stimulated shoot regeneration from callus. All antibiotics under study suppressed rooting of in vitro formed shoots. After five sequential subcultures on kanamycin supplemented medium, antibiotic resistant callus was obtained. To study the impact of kanamycin on resistant callus, total protein content was also studied. Selected callus showed a remarkable increase in callus mass. Antibiotic resistant plants have been selected by screening callus pieces on kanamycin supplemented media. Total protein content increased with subsequent subcultures in kanamycin resistant callus. The kanamycin selected shoots withstood the stability test after 2 months on antibiotic free medium. Plants were raised from the callus, which formed roots in 20 mg dm-3 kanamycin, which was under study.

Keywords: antibiotic resistant; elephant tusk cactus; in vitro selection; plant regeneration; plant tissue culture
Subjects: antibiotics, in vitro culture; auxins; cacti, CAM plants; carbenicillin, organogenesis from callus culture; cefotaxime, in vitro culture; chloramphenicol, in vitro culture; Coryphantha elephantidens; cytokinins; elephant tusk cactus; hygromycin, organogenesis from callus culture; in vitro culture, micropropagation, regeneration; kanamycin, organogenesis from callus culture

Published: March 1, 2001Show citation

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Bhau, B.S., & Wakhlu, A.K. (2001). Effect of Some Antibiotics on the In Vitro Morphogenetic Response from Callus Cultures of Coryphantha Elephantidens. Biologia plantarum, 44(1), 19-24. doi: 10.1023/A:1017905917971.
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