Biologia plantarum 46:357-362, 2003 | DOI: 10.1023/A:1024317900877

Phenylmethylsulphonyl fluoride Inhibits the Formation of Jasmonate-Induced Proteins in Cotyledons of Cucurbita pepo (zucchini)

K.I. Ananieva1, E.D. Ananiev1
1 Institute of Plant Physiology "M. Popov", Bulgarian Academy of Sciences, Sofia, Bulgaria

Phenylmethylsulphonyl fluoride (PMSF), a well known inhibitor of both thiol- and serine-type proteases, in aqueous solutions either alone or with the plant growth regulators, methyl ester of jasmonic acid (MeJA) and N6-benzyl-aminopurine (BAP), significantly inhibited the growth of excised Cucurbita pepo L. (zucchini) cotyledons. SDS-PAGE analysis of the protein profiles showed that PMSF suppressed the gradual decline of the main 20 - 25 kDa polypeptide group and the low molecular mass polypeptides (below 15 kDa) while leupeptine was not able to affect the electrophoretic pattern of cotyledon proteins. On the other hand, in the presence of PMSF, the content of the polypeptides with higher molecular mass including the 97.4 kDa polypeptide and the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (55 kDa) decreased. Besides, when applied together with MeJA, PMSF prevented the appearance of the jasmonate-induced polypeptides (JIPs; 69, 60 and 43 kDa) thus suggesting that JIPs are synthesized from aminoacids released during the breakdown of cotyledon storage proteins.

Keywords: benzylaminopurine; leupeptine; methyl jasmonate; polypeptide profile; storage protein metabolism
Subjects: Cucurbita pepo; cytokinins; gas exchange, ribulose-1,5-bisphosphate carboxylase/oxygenase; jasmonates, jasmonic acid; leupeptine; phenylmethylsulphonyl fluoride; ribulose-1,5-bisphosphate carboxylase/oxygenase; storage protein metabolism; zuccini, cotyledon proteins, jasmonate

Prepublished online: April 1, 2003; Published: November 1, 2003Show citation

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Ananieva, K.I., & Ananiev, E.D. (2003). Phenylmethylsulphonyl fluoride Inhibits the Formation of Jasmonate-Induced Proteins in Cotyledons of Cucurbita pepo (zucchini). Biologia plantarum, 46(3), 357-362. doi: 10.1023/A:1024317900877.
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