Biologia plantarum 50:307-310, 2006 | DOI: 10.1007/s10535-006-0027-z

Phytotoxic effects of Cu, Zn, Cd and Pb on in vitro regeneration and concomitant protein changes in Holarrhena antidysenterica

V. Agrawal1,*, K. Sharma1
1 Department of Botany, University of Delhi, Delhi, India

The nodal explants of in vitro shoots of Holarrhena antidysenterica L. were cultured on Murashige and Skoog's (MS) medium augmented with 15 μM N6-benzyladenine (BA) alone (control) or supplemented with different concentrations (1, 5, 10 and 20 mg dm-3) of CdCl2, CuSO4, Pb(NO3)2 and ZnSO4. The maximum morphogenic response in terms of average shoot number (4.95 ± 0.17) was seen in control. ZnSO4 proved to be less inhibitory in comparison to CuSO4, Pb(NO3)2 and CdCl2. None of the explants cultured on CdCl2 containing medium induced multiple shoots. Maximum protein content [3.80 ± 0.04 mg g-1(f.m.)] was observed in control and slightly less [3.50 ± 0.02 mg g-1(f.m.)] in tissues exposed to 1 mg dm-3 of CuSO4 and minimum [1.00 ± 0.02 mg g-1(f.m.)] in Zn treated (20 mg dm-3) explants. SDS-PAGE analysis of the treated tissues revealed that two new polypeptides (29 and 20 kDa) in response to Cu and Zn treatment, respectively, have been synthesized.

Keywords: N6-benzyladenine; nodal explants; multiple shoots; Murashige and Skoog's medium; protein profile
Subjects: cadmium; copper; cytokinins; heavy metals; Hollarrhena antidisenterica; in vitro culture, callus growth and duration; in vitro culture, regeneration, proliferation, differentiation; lead; nutrient medium, Murashige and Skoog; nutrient medium, Schenk and Hildebrandt (SH); zinc

Received: August 6, 2004; Accepted: June 29, 2005; Published: June 1, 2006Show citation

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Agrawal, V., & Sharma, K. (2006). Phytotoxic effects of Cu, Zn, Cd and Pb on in vitro regeneration and concomitant protein changes in Holarrhena antidysenterica. Biologia plantarum, 50(2), 307-310. doi: 10.1007/s10535-006-0027-z.
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    References

    1. Ali, G., Srivastava, P.S., Iqbal, M.: Effect of cadmium and copper on growth of Bacopa monniera regenerants.-Biol. Plant. 41: 635-639, 1998. Go to original source...
    2. Didierjean, L., Frendo, P., Nasser, W., Genot, G., Marivet, J., Burkad, G.: Heavy metal-responsive genes in maize: identification and comparison of their expression upon various form of abiotic stress-Planta 199: 1-8, 1996. Go to original source...
    3. Hvoslef-Eide, A.K., Corke, F.M.K.: Embryogenesis specific protein changes in birch suspension cultures.-Plant Cell Tissue Organ Cult. 51: 35-41, 1997. Go to original source...
    4. Khan, D.H., Frankland, B.: Effects of cadmium and lead on radish plants with particular reference to movement of metals through soil profile and plant.-Plant Soil 70: 335-345, 1983. Go to original source...
    5. Kumar, R., Sharma, K., Agrawal, V.: In vitro clonal propagation of Holarrhena antidysenterica (L.) Wall. through nodal explants from mature trees.-In Vitro cell dev. Plant. 41: 137-144, 2005.
    6. Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J.: Protein measurement with the Folin phenol reagent.-J. biol. Chem. 193: 265-275, 1951.
    7. Magnard, J.L., Vergne, P., Dumas, C.: Complexity and genetic variability of heat-shock protein expression in isolated maize microspores.-Plant Physiol. 111: 1085-1096, 1996. Go to original source...
    8. Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures.-Physiol. Plant. 15: 473-497, 1962. Go to original source...
    9. Murphy, A., Zhou, J., Goldsbrough, P.B., Taiz L.: Purification and immunological identification of metallothioneins 1 and 2 from Arabidopsis thaliana.-Plant Physiol. 113: 1293-1301, 1997. Go to original source...
    10. Nishizono, H., Watanabe, T., Orii, T., Suzuki, S.: Suppression of inhibitory effects of copper on enzymatic activities by copper-binding substances from Athyrium yokoscense assayed in vitro.-Plant Cell Physiol. 30: 565-569, 1989. Go to original source...
    11. Quzoumidou, G., Moustakas, M., Eleftherou, E.P.: Physiological and ultrastructural effects of cadmium on wheat (Triticum aestivum L.) leaves.-Arch. Environ. Contam. Toxicol. 32: 154-160, 1997.
    12. Purty, R., Agrawal, V., Gupta, S.C.: Induction of a novel boiling stable protein in response to desiccation and ABA treatments in Sesbania sesban var. bicolor leaves.-Biol. Plant. 49: 137-140, 2005. Go to original source...
    13. Raha, S., Roy, S.C.: In vitro plant regeneration in Holarrhena antidysenterica wall. through high frequency axillary shoot proliferation.-In Vitro cell. dev. Biol. Plant 37: 232-236, 2001.
    14. Raha, S., Roy, S.C.: Efficient plant regeneration in Holarrhena antidysenterica Wall. from shoot segment derived callus.-In Vitro cell. dev. biol. Plant 39: 151-155, 2003. Go to original source...
    15. Singla, S.L., Grover, A.: Detection and quantification of a rapidly accumulating and predominant 104 kDa heat shock polypeptide in rice.-Plant Sci. 97: 23-30, 1994. Go to original source...
    16. Steffens, J.C.: The heavy metal-binding peptides of plants.-Annu. Rev. Physiol. Plant mol. Biol. 41: 553-575, 1990. Go to original source...
    17. Sundar, D., Chaitanya, K.V., Reddy, A.R.: Stress-induced proteins in Parthenium argentatum leaves.-Biol. Plant. 46: 313-316, 2003. Go to original source...
    18. Zhou, J., Goldsbrough, P.B.: Functional homologs of fungal metallothionein, genes from Arabidopsis.-Plant Cell 6: 875-884, 1994. Go to original source...
    19. Zhou, J., Goldsbrough, P.B.: Structure, organization and expression of the metallothionein gene family in Arabidopsis.-Mol. gen. Genet. 248: 318-328, 1995. Go to original source...
    20. Zivy, M., Thiellement, H., Tevienna, D., Hofman, J. P.: Study on nuclear and cytoplasmic genome expression in wheat by two dimensional gel electrophoresis.-Theor. appl. Genet. 66: 1-7, 1983. Go to original source...

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