Biologia plantarum 43:157-160, 2000 | DOI: 10.1023/A:1026587904785

Differential Morphogenetic Responses of Cotyledonary Explants of Vigna mungo

G. Franklin1, P.K. Pius2, S. Ignacimuthu1
1 Entomology Research Institute, Loyola College, Chennai-, India e-mail
2 Department of Microbiology, University of Durban-Westville, Durban, RSA

The morphogenetic responses of cotyledonary nodal explants of Vigna mungo (L.) Hepper cv. VBN1 cultured on the same Murashige and Skoog's medium, B5 vitamins, and 13.31 µM N6-benzylaminopurine showed variations in the pattern of multiple shooting and morphology of leaves in dependence on initial explants (presence/absence of cotyledons). The regenerated shoots elongated in the initial medium and most of them rooted in the presence of 2.41 µM indole-3-butyric acid, and flowered in vitro. Rooted plants could be transferred to the field after hardening.

Keywords: apical dominance; in vitro flowering; regeneration; somaclonal variation
Subjects: apical dominance, regeneration in vitro; green gram, regeneration in vitro; in vitro culture, flowering; morphogenetic response, cotyledonary nodal explants; regeneration in vitro; somaclonal variation, cotyledons, flowering in vitro; Vigna mungo

Published: March 1, 2000Show citation

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Franklin, G., Pius, P.K., & Ignacimuthu, S. (2000). Differential Morphogenetic Responses of Cotyledonary Explants of Vigna mungo. Biologia plantarum, 43(1), 157-160. doi: 10.1023/A:1026587904785.
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