Biologia plantarum 50:429-432, 2006 | DOI: 10.1007/s10535-006-0062-9

Effects of short light-dark regimes on in vitro shoot rooting of some fruit tree rootstocks

S. Morini1,*, S. Perrone1
1 Dipartimento di Coltivazione e Difesa delle Specie Legnose "G. Scaramuzzi", Sezione Coltivazioni Arboree, Universita di Pisa, Pisa, Italy

Experiments were carried out to evaluate the effects of 4/2 light-dark cycles (4 h of light followed by 2 h of dark) on the rooting responses of shoots cultivated in vitro of the fruit tree rootstocks GF 677 (peach × almond hybrid), Mr.S. 2/5 (Prunus cerasifera), MM 106 (apple Nothern Spy × Paradise M1) and BA 29 (Cydonia oblonga). Under this light regime rooting percentage of GF 677, Mr.S. 2/5 and MM 106 shoots reached 100 % as in the control treatment (16/8), while in BA 29 shoots, short light-dark cycles increased rooting response by about 65 %. Moreover, the shoots of all rootstocks submitted to the 4/2 cycle showed an appreciable increase in root number and length, and an earlier root emergence of about 4 - 5 d compared to the 16/8 cycle. Finally, rooting percentage of BA 29 shoots submitted to the 4/2 light regime and treated with 0.2 mg dm-3 indolebutyric acid (IBA), was equal to that reported with 0.4 mg dm-3 IBA under the 16/8 regime, indicating that the former light regime also amplified the rhizogenic effect of auxin.

Keywords: Cydonia; Malus; micropropagation; photoperiod; Prunus; rootstocks
Subjects: auxins; Cydonia oblonga; cytokinins; gas exchange; gibberellic acid, gibberellins; in vitro culture, regeneration, proliferation, differentiation; in vitro culture, root cultivation, rooting; nutrient medium, Murashige and Skoog; photoperiod; Prunus cerasifera; root, rooting; rootstock

Received: September 27, 2004; Accepted: September 13, 2005; Published: September 1, 2006Show citation

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Morini, S., & Perrone, S. (2006). Effects of short light-dark regimes on in vitro shoot rooting of some fruit tree rootstocks. Biologia plantarum, 50(3), 429-432. doi: 10.1007/s10535-006-0062-9.
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