Phenolic substances in tissue cultures ofCentaurium erythraea

L. Mebavý

Biologia plantarum 29:81-87, 1987 | DOI: 10.1007/BF02878153

Phenolic compounds l,2,3-trihydroxy-5-methoxyxanthone, l-hydroxy-3,5,6,7, 8-pentamethoxyxanthone, and l,8-dihydroxy-2,3,4,6-tetramethoxyxanthone predominate in the callus tissue ofCentaurium erythraea, their content increasing with culture age. By contrast, the contents of the derivatives of cinnamic, chlorogenic, and ferulic acids decrease or do not change. In the cell suspension culture ofC. erythraea a larger amount of xanthones is synthesized than in the callus from which the suspension culture was derived. The content of phenolic acids is lower in the suspension culture than in the callus, but a larger number of low-molecular-mass...

Biotransformation of 2-(4-methoxybenzyl)-l-cyclohexanone by cell cultures ofSolatium aviculare

Z. Wimmer, T. Macek, T. Vaněk, L. Streinz, M. Romaňuk

Biologia plantarum 29:88-93, 1987 | DOI: 10.1007/BF02878154

During the search for compounds with insect juvenile hormone activity, the biotransformation of 2-(4-methoxybenzyl)-l-cyclohexanone, of 2-(4-methoxybenzyl)-l-cyclohexanone ethylene acetal and of both isomers of 2-(4-methoxybenzyl)-l-cyclohexanol by plant cells was examined. The compounds were metabolized by cell suspension culture of Solatium aviculare Forst. The reaction conditions were optimized and the metabolic products isolated and identified. A scheme of biotransformation pathway has been proposed.

Book reviews

T. Gichner

Biologia plantarum 29:93, 1987 | DOI: 10.1007/BF02878155

Book reviews

T. Gichner

Biologia plantarum 29:93, 1987 | DOI: 10.1007/BF02878156

Histochemical IAA-oxidase localization in the shoot of wheat

Z. Zmrhal, Frideta Seidlová, Ivana Macháčková

Biologia plantarum 29:94-97, 1987 | DOI: 10.1007/BF02878157

A histochemical method for the determination of IAA-oxidase has been used in sections of various aerial parts of winter wheat plants. High IAA-oxidase activity was localized in the cell walls of sclerenchyma near the periphery of the stem, in the vascular bundle sheath of sclerenchyma and in xylem, both in the stem and in the leaf. The cell wall-bound IAA-oxidase activity therefore appeared in lignifying tissues. The staining was very weak or absent in the cell walls of parenchyma tissues and phloem. The positive reaction of the cytosol at the bulbous ends of guard cells and in the leaf primordia is presumed to be due to cytosolic IAA-oxidase. These...

Purification and immunohistochemical localization of the auxin binding site i protein from maize coleoptiles

M. Löbler, D. Klämbt

Biologia plantarum 29:98-103, 1987 | DOI: 10.1007/BF02878158

An auxin binding protein fraction prepared by means of affinity chromatography on 2-OH-3,5-diiodobenzoic acid-Sepharose and gel filtration was used as antigen. The obtained rabbit antisera contained antibodies against the auxin, binding protein (ABP) and several contaminating proteins (nonABP). The nonABP could be separated on an appropriate affinity matrix omitting the TIBA analogue. After their immobilization on Sepharose antibodies directed towards contaminating, the proteins were isolated and immobilized, too. This IgGanti nonABP-Sepharose retains almost all contaminating proteins present in the specific eluates of the auxin affinity matrix. In...

Book review

L. Šindelář

Biologia plantarum 29:103, 1987 | DOI: 10.1007/BF02878159

Changes in the relationship between growth of leaves and stem in cabbage caused by treatments with growth retardants

Svetlana Koshtjchowa, G. Meinl, H. Münnich, H. Göring

Biologia plantarum 29:104, 1987 | DOI: 10.1007/BF02878160

A cabbage variety with particularly strongly developed leaves and extremely shortened stems (tight heads) was treated during the first year of its development with growth retardants (CCC and CEPA, Ethephon) at different concentrations. A 4-fold treatment with CCC, CEPA or a mixture of both proved to be most effective Leaf growth was inhibited by this treatment (head mass decreased) but stem growth increased. Only in treated plants the bud of the stem apex could break through the leaf sheath and develop generative stem. The results are discussed as examples of correlative relations between leaf and stem growth.

Book review

J. Tupý

Biologia plantarum 29:109, 1987 | DOI: 10.1007/BF02878161

Non-Decarboxylating transformation of indol-3-ylacetic acid in apple seeds

Katarzyna Dziewanowska, S. Lewak

Biologia plantarum 29:110-117, 1987 | DOI: 10.1007/BF02878162

An enzyme extract from apple(Pyrus malus Borb.) seeds which causes the disappearance of free indol-3-ylacetic acid (IAA) requires the presence of oxygen, but is not inhibited by cyanide. Using 1-¹⁴C-IAA it has been demonstrated that the IAA transformation is not accompanied by its decarboxylation. Decarboxylating IAA oxidase is absent during the whole period of apple seed cold stratification. Free IAA has not been detected in dormant apple seeds and in seeds stratified at low temperature. It appears during stratification at 25 °C. Ethyl ester of IAA and indol-3-ylacetyl aspartate have been identified in dormant and after-ripened seeds....

Effect of gibberellic acid on the uptake and loss of inorganic phosphate from sweet potato slices

G. F. Israelstam

Biologia plantarum 29:118-122, 1987 | DOI: 10.1007/BF02878163

Uptake and loss of irorganic phosphate was determined in sweet potato slices 'aged' in the presence or absence of gibberellic acid. Concentrations of uptake medium were varied from 0.1 to 1.2 mM. Slices, aged in gibberellic acid, took up considerably less phosphate, at all concentrations than control, whereas efflux from GA₃ treated discs was greater than control. Whether treated or untreated, kinetics proved to be biphasic hyperbolas. The results suggested an alteration in membrane permeability induced by gibberellic acid.

The effect of indol-3-yllactic acid on maize seedling growth

Eva ZelenÁ

Biologia plantarum 29:123-128, 1987 | DOI: 10.1007/BF02878164

D,L-indol-3-yllactie acid was applied at concentrations 0.2 - 20 mg 1^-1 (10^-6-10^-4M) to the roots of 3-d-old intact maize seedlings grown in the dark and in the light. By day 3 after application, ILA at lower concentrations (0.2-1 mg l^-1) caused slight increase in the mass of etiolated plants, mainly roots. Shoot and root length was not increased. ILA at concentrations equal to, or greater than, 2 mg 1^-1 reduced the length of the main roots and from 10 mg 1^-1 on also the length of the shoots. In contrast, root mass was decreased by higher ILA concentrations to a lesser extent than shoot...

Book review

J. Tupý

Biologia plantarum 29:128, 1987 | DOI: 10.1007/BF02878165

The effect of cold storage at 4 °C on the Rooting of chestnut cuttings

T. Diaz, J. L. G. Mantilla, E. Vieitez

Biologia plantarum 29:129-133, 1987 | DOI: 10.1007/BF02878166

This article reports the effect of cold storage at 4 °C from December to April on chestnut cuttings. Rooting was found to increase after four months preceded by treatment with 4000 mg l^-1 IBA.
ThePhaseolus test of the biological activity of extracts of the cuttings showed the inhibition exerted by the acid fraction of the fresh (December) cuttings to have been replaced by a rootpromoting effect in cuttings stored for 4 months at low temperature. Cold storage thus seems to favour rooting.

Sequential analysis of nuclei transformation in stem nodulation sites ofSesbania rostrata Bbem (Leguminosae) during infection by specificrhizobium

E. Duhoux, C. Le Coq, C. Guervin

Biologia plantarum 29:134-142, 1987 | DOI: 10.1007/BF02878167

This paper is a sequential analysis of qualitative and quantitative nuclear evolution inSesbania rostrata (Leguminosae) stem nodules. Before infection, the nuclei of the root primordia (nodulation site) cells show a 2G level of DNA. Immediately upon infection, the cells cease their mitotic activity and the nuclei begin synthesizing DNA up to a 32C level after the onset of the infection. The increase in the diameter of the nuclei of the infected cells is concomitant with the rise in the level of DKA. In the final phase of the evolution of these nodules, the nuclei of the infected cells undergo degenerative changes.

Book review

F. Kunc

Biologia plantarum 29:142, 1987 | DOI: 10.1007/BF02878168

Soluble proteins inNicotiana plumbaginifolia crown-gall tumors

B. Vyskot, Viera Kuhrová

Biologia plantarum 29:143-149, 1987 | DOI: 10.1007/BF02878169

Electrophoretic separations of soluble proteins extracted from crown-gall tumors, normal calli, and leavesof Nicotiana plumbaginifolia were performed in order to detect pathogenic changes caused byAgrobacterium tumefaciens transformation. These analyses showed the appearance of new low-molecular polypeptides both in crown-gall tumors and in normal calli. The SDS-polyacrylamide gel electrophoresis as well as the gel electrophoresis in non-denaturing conditions were used to characterize their properties. At least some of them corresponded to the pathogenesis-related proteins reported inN. tabacum. Isoenzyme patterns of peroxidases...

Changes in the activity of phosphogluconate dehydrogenase and its regulation in tobacco infected with PVY

L. Šindelář, Milada Šindelářová

Biologia plantarum 29:150-154, 1987 | DOI: 10.1007/BF02878170

The content and changes in the activity of phosphogluconate dehydrogenase were followed in leaf tissues of tobacco plants infected with the potato virus Y (PVY) in the acute phase of infection. The activity of the enzyme was higher in virus-infected tissues during the entire experimental period compared with the values found in healthy control plants in both crude homogenate and partially purified enzyme preparation. The courses of the activity curves of both the crude extract and partially purified enzyme preparations were consistent and correlated with the reproduction curve of the virus. These results suggest that increased activity of the enzyme...

Book review

Jarmila Solárová

Biologia plantarum 29:154, 1987 | DOI: 10.1007/BF02878171

Book review

T. Gichner

Biologia plantarum 29:155, 1987 | DOI: 10.1007/BF02878172

Book review

Ingrid Tichá

Biologia plantarum 29:155, 1987 | DOI: 10.1007/BF02878173

Book review

J. Krekule

Biologia plantarum 29:155-156, 1987 | DOI: 10.1007/BF02878174

Book review

Z. Šesták

Biologia plantarum 29:156-157, 1987 | DOI: 10.1007/BF02878175

Book review

Ingrid Tichá

Biologia plantarum 29:157, 1987 | DOI: 10.1007/BF02878176

Book review

Jarmila Solárová

Biologia plantarum 29:157-158, 1987 | DOI: 10.1007/BF02878177

Book review

C. Záruba

Biologia plantarum 29:158, 1987 | DOI: 10.1007/BF02878178

In memorian of professor hitoshi kihara (1893-1986)

Singo Nakazawa

Biologia plantarum 29:159-160, 1987 | DOI: 10.1007/BF02878179