Biologia plantarum 52:413-422, 2008 | DOI: 10.1007/s10535-008-0085-5

Production of reactive oxygen species and development of antioxidative systems during in vitro growth and ex vitro transfer

P. Baťková1,2, J. Pospíšilová1,*, H. Synková1
1 Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Prague, Czech Republic
2 Faculty of Science, Charles University Prague, Prague, Czech Republic

Ex vitro transfer is often stressful for in vitro grown plantlets. Water stress and photoinhibition, often accompanying the acclimatization of in vitro grown plantlets to ex vitro conditions, are probably the main factors promoting production of reactive oxygen species (ROS) and in consequence oxidative stress. The extent of the damaging effects of ROS depends on the effectiveness of the antioxidative systems which include low molecular mass antioxidants (ascorbate, glutathione, tocopherols, carotenoids, phenols) and antioxidative enzymes (superoxide dismutase, ascorbate peroxidase, catalase, glutathione reductase, monodehydroascorbate reductase, dehydroascorbate reductase). This review is focused on ROS production and development of antioxidative system during in vitro growth and their further changes during ex vitro transfer.

Keywords: ascorbate; ascorbate peroxidase; carotenoids; catalase; dehydroascorbate reductase; glutathione; glutathione reductase; malondialdehyde; peroxidase; superoxide dismutase
Subjects: antioxidants, antioxidant enzymes; ascorbate peroxidase; carotenoids; catalase; dehydroascorbate reductase; ex vitro transfer; glutathione reductase; in vitro culture, growth control; malondialdehyde; peroxidase; photoinhibition; superoxide dismutase (SOD); water stress

Received: December 7, 2007; Accepted: January 31, 2008; Published: September 1, 2008Show citation

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Baťková, P., Pospíšilová, J., & Synková, H. (2008). Production of reactive oxygen species and development of antioxidative systems during in vitro growth and ex vitro transfer. Biologia plantarum, 52(3), 413-422. doi: 10.1007/s10535-008-0085-5.
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