Biologia plantarum 52:557-560, 2008 | DOI: 10.1007/s10535-008-0110-8

In vitro plant regeneration from leaf explants of Ophiorrhiza japonica

G.-Y. Kai1,*, L.-M. Dai1, X.-Y. Mei1, J.-G. Zheng2, W. Wang1, Y. Lu1, Z.-Y. Qian1, G.-Y. Zhou1
1 Laboratory of Plant Biotechnology, College of Life and Environment Sciences, Shanghai Normal University, Shanghai, P.R. China
2 Laboratory of Quality Biotechnology, School of Life Sciences, Fujian Agriculture and Forest University, Fuzhou, P.R. China

An efficient in vitro plant regeneration system from leaves of Ophiorrhiza japonica Blume was established for the first time. Callus formation rate was more than 90.4 % from leaf segments on Murashige and Skoog (MS) supplemented with either α-naphthaleneacetic acid (NAA) alone or in combination with 6-benzyladenine (BA). The highest shoot regeneration (78.9 %) was achieved on MS medium containing 2.0 mg dm-3 BA and 0.2 mg dm-3 NAA, with an average of 9.4 shoots developed per leaf segment. Shoot regeneration was also improved when the leaf explants were cultured in MS basal medium supplemented with 0.5 % (m/v) polyvinylpyrrolidone (PVP). The leaf explants from seedlings with age of about 18-27 d showed the highest shoot regeneration. The regenerated shoots were rooted on half-strength basal MS medium supplemented with 0.5 mg dm-3 indole-3-butyric acid (IBA), which averagely produced 24.8 roots per shoot. The plantlets were transferred to soil, where 100 % survived after 1 month of acclimatization.

Keywords: camptothecin; growth regulators; medicinal plant; shoot regeneration
Subjects: auxins; camptothecin; in vitro culture, regeneration; medicinal plants; nutrient medium, Murashige and Skoog (MS); Ophiorrhiza japonica

Received: February 16, 2007; Accepted: January 5, 2008; Published: September 1, 2008Show citation

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Kai, G.-Y., Dai, L.-M., Mei, X.-Y., Zheng, J.-G., Wang, W., Lu, Y., Qian, Z.-Y., & Zhou, G.-Y. (2008). In vitro plant regeneration from leaf explants of Ophiorrhiza japonica. Biologia plantarum, 52(3), 557-560. doi: 10.1007/s10535-008-0110-8.
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