Biologia plantarum 59:793-796, 2015 | DOI: 10.1007/s10535-015-0546-6

Effect of low temperature on profilins and ADFs transcription and actin cytoskeleton reorganization in Arabidopsis

T. T. Fan1,*, J. J. Ni1, W. C. Dong1, L. Z. An2, Y. Xiang2, S. Q. Cao1
1 School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei, Anhui, P.R. China
2 School of Life Sciences, Lanzhou University, Lanzhou, P.R. China

In the present study, we found that the expression patterns of the vegetative profilins and actin depolymerizing factors (ADFs) were specifically altered under low temperature (17 °C) stress using a real-time PCR. Our results show that also reorganization of the actin cytoskeleton was triggered by a low temperature. Facilitation of microfilament (MF) assembly by phalloidin treatment resulted in an enhanced low temperature stress tolerance, whereas blocking MF assembly with latrunculin B resulted in enhanced low temperature stress sensitivity. Our results show that the specific members of the vegetative profilins and ADFs might participate in regulating the response of plants to a low temperature stress, and the actin cytoskeleton is vital for the tolerance of Arabidopsis seedlings to low temperature stress.

Keywords: latrunculin B; microfilaments; phalloidin; stress tolerance
Subjects: temperature - low; actin; cytoskeleton; profilin; gene expression
Species: Arabidopsis thaliana

Received: September 23, 2014; Revised: April 2, 2015; Accepted: May 6, 2015; Published: December 1, 2015Show citation

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Fan, T.T., Ni, J.J., Dong, W.C., An, L.Z., Xiang, Y., & Cao, S.Q. (2015). Effect of low temperature on profilins and ADFs transcription and actin cytoskeleton reorganization in Arabidopsis. Biologia plantarum, 59(4), 793-796. doi: 10.1007/s10535-015-0546-6.
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    References

    1. Aderem, A.: Signal transduction and the actin cytoskeleton: the roles of MARCKS and profilin. - Trends Plant Sci. 17: 438-443, 1992. Go to original source...
    2. Bamburg, J.R.: Proteins of the ADF/cofilin family: essential regulators of actin dynamics. - Annu. Rev. cell. dev. Biol. 15: 185-230, 1999. Go to original source...
    3. De Silva, K., Laska, B., Brown, C., Sederoff, H.W., Khodakovskaya, M.: Arabidopsis thaliana calciumdependent lipid-binding protein (AtCLB): a novel repressor of abiotic stress response. - J. exp. Bot. 62: 2679-2689, 2011. Go to original source...
    4. Egierszdorff, S., Kacperska, A.: Low temperature effects on growth and actin cytoskeleton organization in suspension cells of winter oilseed rape. - Plant Cell Tissue Organ Cult. 65: 149-158, 2001. Go to original source...
    5. Huang, S.L., Jin, L.F., Du, J.Z., Li, H., Zhao, Q., Ou, G.S., Ao, G.M., Yuan, M.: SB401, a pollen-specific protein from Solanum berthaultii, binds to and bundles microtubules and F-actin. - Plant J. 51: 406-418, 2007. Go to original source...
    6. Kandasamy, M.K., McKinney, E.C., Meagher, R.B.: Plant profilin isovariants are distinctly regulated in vegetative and reproductive tissues. - Cell motiv. Cytol. 52: 22-32, 2002. Go to original source...
    7. Machesky, L.M., Pollard, T.D.: Actin associated proteins: profilin. - In: Kreis, T., Vale, R. (ed.): Guidebook to the Cytoskeletal and Motor Proteins. Pp. 66-68. Oxford University Press, New York 1993.
    8. Orvar, B.L., Sangwan, V., Omann, F., Dhindsa, R.S.: Early steps in cold sensing by plant cells: the role of actin cytoskeleton and membrane fluidity. - Plant J. 23: 785-794, 2000. Go to original source...
    9. Ouellet, F., Carpentier, E., Cope, M.J.T.V., Monroy, A.F., Sarhan, F.: Regulation of a wheat actin-depolymerizing factor during cold acclimation. - Plant Physiol. 125: 360-368, 2001. Go to original source...
    10. Pokorna, J., Schwarzerova, K., Zelenkova, S., Petrasek, J., Janotova, I., Capkova, V., Opatrny, Z.: Sites of actin filament initiation and reorganization in cold treated tobacco cells. - Plant Cell Environ. 27: 641-653, 2004. Go to original source...
    11. Ruzicka, D.R., Kandasamy, M.K., McKinney, E.C., Burgos-Rivera, B., Meagher, R.B.: The ancient subclasses of Arabidopsis ACTIN DEPOLYMERIZING FACTOR genes exhibit novel and differential expression. - Plant J. 52: 460-472, 2007. Go to original source...
    12. Sangwan, V., Foulds, I., Singh, J., Dhindsa, R.S.: Cold induction of Brassica napus gene, BN115, is mediated by structural changes in the membrane and cytoskeleton and requires Ca2+ influx. - Plant J. 27: 1-12, 2001. Go to original source...
    13. Voigt, B., Timmers, T., Samaj, J., Muller, J., Baluska, F., Menzel, D.: GFP-FABD2 fusion construct allows in vivo visualization of the dynamic actin cytoskeleton in all cells of Arabidopsis seedlings. - Eur. J. cell. Biol. 84: 595-608, 2005. Go to original source...
    14. Wang, C., Zhang, L., Yuan, M., Ge, Y., Liu, Y., Fan, J., Ruan, Y., Cui, Z., Tong, S., Zhang, S.: The microfilament cytoskeleton plays a vital role in salt and osmotic stress tolerance in Arabidopsis. - Plant Biol. 12: 70-78, 2009. Go to original source...
    15. Wasteneys, G.O., Yang, Z.: New views on the plant cytoskeleton. - Plant Physiol. 136: 3884-3891, 2004. Go to original source...

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