Biologia plantarum 62:786-792, 2018 | DOI: 10.1007/s10535-018-0820-5

Molecular cloning and characterization of a PR-5 like protein gene from Brassica campestris ssp. chinensis

C. Liu1,2, H. L. Liu1,2, Y. Wang1,2, D. Hu1,2, D. Xiao1,2, C. W. Zhang1,2, X. L. Hou1,2, Y. Li1,2,*
1 College of Horticulture, Nanjing Agricultural University, Nanjing, P.R. China
2 State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing, P.R. China

Downy mildew caused by Hyaloperonospora parasitica is a serious fungal disease in non-heading Chinese cabbage (Brassica campestris L. ssp. chinensis Makino). Pathogenesis-related 5 (PR-5) genes play an important role in plant resistance to disease invasion. In this study, a gene encoding pathogenesis-related 5-like (PR-5L) protein, named BcPR-5L, was successfully cloned from non-heading Chinese cabbage. The cDNA sequence of BcPR-5L is 747 bp in length. It encoded a protein of molecular mass of 25.78 kDa, an isoelectric point of 4.42, and containing 248 amino acids. Multiple sequence alignment indicated that BcPR-5L protein was highly homologous to other PR-5L proteins identified in 13 different species, with the highest homology to Brassica rapa. We analyzed the subcellular localization of BcPR- 5L protein by using onion epidermal cells and found that it is localized in the membrane. Real time quantitative PCR analyses revealed that the expression of BcPR-5L gene was significantly upregulated after H. parasitica infection, and the expression in the resistant cultivar was higher than that in the susceptible cultivar. In summary, our data suggest that BcPR-5L gene may play an important role in the resistance of non-heading Chinese cabbage to H. parasitica infection.

Keywords: Hyaloperonospora parasitica; non-heading Chinese cabbage; onion; subcellular localization
Subjects: pathogenesis related gene; pathogenesis-related proteins; subcellular localization; amino acid sequences; phylogenetic tree; Chinese cabbage
Species: Brassica campestris

Received: September 27, 2017; Revised: April 17, 2018; Accepted: April 25, 2018; Published: August 1, 2018Show citation

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Liu, C., Liu, H.L., Wang, Y., Hu, D., Xiao, D., Zhang, C.W., Hou, X.L., & Li, Y. (2018). Molecular cloning and characterization of a PR-5 like protein gene from Brassica campestris ssp. chinensis. Biologia plantarum, 62(4), 786-792. doi: 10.1007/s10535-018-0820-5.
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