Biologia plantarum 52:101-104, 2008 | DOI: 10.1007/s10535-008-0018-3

Endogenous abscisic acid and protein contents during seed development of Araucaria angustifolia

V. Silveira1, C. Santa-Catarina2, T. S. Balbuena2, F. M. S. Moraes3, C. A. O. Ricart3, M. V. Sousa3, M. P. Guerra4, W. Handro2, E. I. S. Floh2,*
1 Biotechnology Laboratory, CBB-UENF, Campos dos Goytacazes-Rio de Janeiro, Brazil
2 Department of Botany, IB-University of São Paulo, CP 11461, São Paulo-SP, Brazil
3 Department of Cell Biology, IB-University of Brasilia, Brasília-DF, Brazil
4 Department of Plant Science, CCA-Federal University of Santa Catarina, Florianópolis-SC, Brazil

This paper describes a proteome analysis and changes in endogenous abscisic acid (ABA) contents during seed development of Araucaria angustifolia (Bert.) O. Ktze. Megagametophytes and embryonic axis tissues exhibited a similar ABA variation pattern during seed development, reaching maximum values at the pre-cotyledonary stage. The embryonic axis protein content increased until the cotyledonary stage with following stabilization at mature seed. The two-dimensional electrophoresis at the torpedo developmental stage showed approximately 230 polypeptides against 340 in the mature stage. Peptide mass fingerprinting analyses identified three polypeptides, corresponding to an AtSAC4, a late embryogenesis abundant (LEA) and a storage protein, respectively.

Keywords: comparative proteomic; conifer; LEA; plant proteome; somatic embryogenesis; storage protein; zygotic embryo
Subjects: abscisic acid (ABA); Araucaria angustifolia; in vitro culture, somatic embryogenesis; proteins; seed development; zygotic embryogenesis

Received: January 18, 2006; Accepted: October 17, 2006; Published: March 1, 2008Show citation

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Silveira, V., Santa-Catarina, C., Balbuena, T.S., Moraes, F.M.S., Ricart, C.A.O., Sousa, M.V., ... Floh, E.I.S. (2008). Endogenous abscisic acid and protein contents during seed development of Araucaria angustifolia. Biologia plantarum, 52(1), 101-104. doi: 10.1007/s10535-008-0018-3.
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