biologia plantarum

Biologia plantarum 54:691-696, 2010 | DOI: 10.1007/s10535-010-0122-z

An EST-SSR marker linked with yellow rust resistance in wheat

S. Ercan¹, F. Ertugrul², Y. Aydin¹, F. S. Akfirat³, S. Hasancebi², K. Akan⁴, Z. Mert⁴, N. Bolat⁵, O. Yorgancilar⁵, A. Altinkut-Uncuoglu^2,*

¹ Faculty of Science and Letters, Department of Biology, Marmara University, Kadikoy, Istanbul, Turkey

² TUBITAK, MRC, Genetic Engineering and Biotechnology Institute, Gebze, Kocaeli, Turkey

³ Faculty of Science, Department of Biology, Gebze Institute of Technology, Gebze, Kocaeli, Turkey

⁴ Field Crop Research Institute, Lodumlu, Ankara, Turkey

⁵ Anatolian Agricultural Research Institute, Eskisehir, Turkey

Expressed sequenced tags containing simple sequence repeats (EST-SSRs) were used to identify molecular markers associated with yellow rust resistance in wheat (Triticum aestivum L.). A cross between yellow rust resistant (PI178383) and susceptible (Harmankaya99) wheat genotypes was performed and respective DNA pools from the resistant and susceptible F₂ seedlings were constructed. 78 EST-SSR primers were used for bulked segregant analysis and one EST-SSR marker (Pk54), identified as 200 bp fragment, was present in the resistant parent and resistant F₂ hybrids but not in the susceptible ones. 108 wheat genotypes differing in yellow rust resistance were screened with Pk54 and 68 % of the wheat genotypes, known to be yellow rust resistant, had the Pk54 marker, further suggesting that the presence of this marker correlates with yellow rust resistance.

Keywords: breeding; Puccinia striiformis f. sp. tritici; Triticum aestivum

Subjects: expressed sequence tag; Puccinia striiformis; resistance marker; simple sequence repeats (SSR); Triticum aestivum; wheat; yellow rust

Received: January 24, 2009; Accepted: October 5, 2009; Published: December 1, 2010Show citation