Biologia plantarum 55:183-186, 2011 | DOI: 10.1007/s10535-011-0027-5

Influence of irradiance on chlorophyll synthesis in Picea abies calli cultures

R. Balážová1,*, A. Blehová1, V. Demko1, K. Breznenová1, J. Hudák1
1 Department of Plant Physiology, Faculty of Natural Sciences, Comenius University, Bratislava, Slovakia

Dark-grown seedlings of Picea abies (L) Karst. are able to accumulate the highest amounts of chlorophyll (Chl) and its precursor protochlorophyllide (Pchlide) in all Pinaceae, but calli derived from 14-d-old green cotyledons of P. abies are completely white during the cultivation in the dark. Pchlide reduction is catalysed in the dark by light-independent protochlorophyllide oxidoreductase (DPOR). This enzyme complex consists of three protein subunits ChlL, ChlN and ChlB, encoded by three plastid genes chlL, chlN and chlB. Using semiquantitative RT-PCR, we observed very low expression of chlLNB genes in dark-grown calli. It seems, that chlLNB expression and thus Chl accumulation could be modulated by light in P. abies calli cultures. This hypothesis is supported by the fact, that we observed low contents of glutamyl-tRNA reductase and Flu-like protein, which probably affected Chl biosynthetic pathway at the step of 5-aminolevulinic acid formation. ChlB subunit was not detected in dark-grown P. abies calli cultures. Our results indicated limited ability to synthesize Chl in callus during cultivation in the dark.

Keywords: chlorophyllide; light-independent protochlorophyllide oxidoreductase; Norway spruce; plastid gene expression; protochlorophyllide reduction

Received: July 6, 2009; Accepted: April 5, 2010; Published: March 1, 2011Show citation

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Balážová, R., Blehová, A., Demko, V., Breznenová, K., & Hudák, J. (2011). Influence of irradiance on chlorophyll synthesis in Picea abies calli cultures. Biologia plantarum, 55(1), 183-186. doi: 10.1007/s10535-011-0027-5.
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    References

    1. Armstrong, G.A.: Greening in the dark: light-independent chlorophyll biosynthesis from anoxygenic photosynthetic bacteria to gymnosperms. - J. Photochem. Photobiol. B 43: 87-100, 1998. Go to original source...
    2. Demko, V., Pavlovič, A., Valková, D., Slováková, Ľ., Grimm, B., Hudák, J.: A novel insight into the regulation of lightindependent chlorophyll biosynthesis in Larix decidua and Picea abies seedlings. - Planta 230: 165-176, 2009. Go to original source...
    3. Falciatore, A., Merendino, L., Barneche, F., Ceol, M., Meskauskiene, R., Apel, K., Rochaix, J.-D.: The FLP proteins act as regulators of chlorophyll synthesis in response to light and plastid signals in Chlamydomonas. - Genes Dev. 19: 176-187, 2005. Go to original source...
    4. Fujita, Y.: Protochlorophyllide reduction: a key step in the greening of plants. - Plant Cell Physiol. 37: 411-421, 1996. Go to original source...
    5. Fujita, Y., Takagi, H., Hase, T.: Cloning of the gene encoding a protochlorophyllide reductase: the physiological significance of the co-existence of light-dependent and - independent protochlorophyllide reduction systems in the cyanobacterium Plectonema boryanum. - Plant Cell Physiol. 39: 177-185, 1998. Go to original source...
    6. Fujita, Y., Bauer, C.E.: The light-independent protochlorophyllide reductase: a nitrogenase-like enzyme catalyzing a key reaction for greening in the dark. - In: Kadish, K.M., Smith, K.M., Guilard, R. (ed.): The Porphyrin Handbook. Vol. 13. Pp. 109-156. Elsevier, Boston 2003. Go to original source...
    7. Hudák, J., Gálová, E., Zemanová, L.: Plastid morphogenesis. - In: Pessarakli, M. (ed.): Handbook of Photosynthesis. Pp. 221-246. CRC Press, Taylor and Francis Group, Boca Raton 2005.
    8. Ilag, L.L., Kumar, A.M., Soll, D.: Light regulation of chlorophyll biosynthesis at the level of 5-aminolevulinate formation in Arabidopsis. - Plant Cell 6: 256-275, 1994. Go to original source...
    9. Karpinska, B., Karpinsky, S., Hällgren, J.-E.: The chlB gene encoding a subunit of light-independent protochlorophyllide reductase is edited in chloroplasts of conifers. - Curr. Genet. 31: 343-347, 1997. Go to original source...
    10. Kruse, E., Mock, H.P., Grimm, B.: Reduction of coproporphyrinogen oxidase level by antisense RNA synthesis leads to deregulated gene expression of plastid proteins and affects the oxidative defense system. - EMBO J. 14: 3712-3720, 1995. Go to original source...
    11. Kruse, E., Grimm, B., Beator, J., Kloppstech, K.: Developmental and circadian control of the capacity for delta-aminolevulinic acid synthesis in green barley. - Planta 202: 235-241, 1997. Go to original source...
    12. Kusumi, J., Sato, A., Tachida, H.: Relaxation of functional constraint on light-independent protochlorophyllide oxidoreductase in Thuja. - Mol. Biol. Evol. 23: 941-948, 2006. Go to original source...
    13. Ladygin, V.G., Bondarev, N.I., Semenova, G.A., Smolov, A.A., Reshetnyak, O.V., Nosov, A.M.: Chloroplast ultrastructure, photosynthetic apparatus activities and production of steviol glycosides in Stevia rebaudiana in vivo and in vitro. - Biol. Plant. 52: 9-16, 2008. Go to original source...
    14. Lichtenthaler, H.K.: Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. - Methods Enzymol. 148: 350-382, 1987. Go to original source...
    15. Mariani, P., De Carli, M.E., Rascio, N., Baldan, B., Casadoro, G., Gennari, G., Bodner, M., Larcher, W.: Synthesis of chlorophyll and photosynthetic competence in etiolated and greening seedlings of Larix decidua as compared with Picea abies. - J. Plant Physiol. 137: 5-14, 1990. Go to original source...
    16. Meskauskiene, R., Nater, M., Goslings, D., Kessler, F., Op den Camp, R., Apel, K.: FLU: a negative regulator of chlorophyll biosynthesis in Arabidopsis thaliana. - Proc. nat. Acad. Sci. USA 98: 12826-12831, 2001. Go to original source...
    17. Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures. - Plant Physiol. 15: 473-497, 1962. Go to original source...
    18. Nogaj, L.A., Srivastava, A., Van Lis, R., Beale, S.I.: Cellular levels of glutamyl-tRNA reductase and glutamate-1-semialdehyde aminotransferase do not control chlorophyll synthesis in Chlamydomonas reinhardtii. - Physiol. Plant 139: 389-396, 2005. Go to original source...
    19. Salajová, T., Jásik, J., Salaj, J.: In Vitro Cultures of Conifers. - Veda, Bratislava 1998.
    20. Shi, C.L., Shi, X.M.: Expression switching of three genes encoding light-independent protochlorophyllide in Chlorella protothecoides. - Biotechnol. Lett 28: 261-265, 2006. Go to original source...
    21. Schoefs, B.: The light-dependent and light-independent reduction of protochlorophyllide a to chlorophyllide a. - Photosynthetica 36: 481-496, 1999. Go to original source...
    22. Skinner, J.S., Timko, M.P.: Differential expression of genes encoding the light-dependent and light-independent enzymes for protochlorophyllide reduction during development in loblolly pine. - Plant Mol. Biol. 39: 577-592, 1999. Go to original source...
    23. Spano, A.J., He, Z.H., Timko, M.P.: NADPH:protochlorophyllide oxidoreductases in white pine (Pinus strobus) and loblolly pine (P. teada). - Mol. gen. Genet. 236: 86-95, 1992.
    24. Suzuki, J.Y., Bauer, C.E.: Light-independent chlorophyll biosynthesis: involvement of the chloroplast gene chlL (frxC). - Plant Cell 4: 929-940, 1992. Go to original source...
    25. Tanaka, R., Tanaka, A.: Tetrapyrrole biosynthesis in higher plants. - Annu. Rev. Plant Biol. 58: 321-346, 2007. Go to original source...
    26. Triboush, S.O., Danilenko, N.G., Davydenko, O.G.: A method for isolation of chloroplast DNA and mitochondrial DNA from sunflower. - Plant mol. Biol. Rep. 16: 183-189, 1998. Go to original source...
    27. Von Wettstein, D., Gough, S., Kannangara, C.G.: Chlorophyll biosynthesis. - Plant Cell 7: 1039-1057, 1995. Go to original source...

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