Biologia plantarum 56:551-559, 2012 | DOI: 10.1007/s10535-011-0226-0

Changes in apoplast protein pattern suggest an early role of cell wall structure remodelling in flagellin-triggered basal immunity

E. Szabó1, Á. Szatmári1, É. Hunyadi-Gulyás2, E. Besenyei1, L. R. Zsiros1, Z. Bozsó1, P. G. Ott1,*
1 Plant Protection Institute, Hungarian Academy of Sciences, Budapest, Hungary
2 Biological Research Center, Hungarian Academy of Sciences, Szeged, Hungary

The leaf apoplast is a dynamic compartment in contact with plant pathogenic bacteria after infection. Among the very first interaction events is the receptor-mediated perception of bacterial surface molecules such as flagellin or other conserved microbe-associated molecular patterns (MAMPs). Apoplast proteins likely play a role in basal resistance (BR) or pattern-triggered immunity (PTI). Here, a proteomic approach was carried out on water soluble - potentially the most mobile - apoplast proteins from flagellin-treated tobacco (Nicotiana tabacum) leaves. As the quickness of BR/PTI seems crucial for its efficacy, samples were taken as early as 2.5 and 7 h post inoculation. Proteins were separated by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and identified by liquid chromatography tandem mass spectrometry (LC-MS/MS). Forty-nine different proteins from 28 protein spots changed in their density compared to the water-inoculated control. Eleven protein spots appeared de novo in response to EBR induction. There are glycohydrolases and redox-active proteins besides pathogenesis-related proteins among them, predicting plant cell wall structural modifications and more direct antimicrobial effectors as earliest changes related to BR/PTI.

Keywords: basal resistance; glycohydrolases; pathogenesis-related proteins; proteomics; tobacco
Subjects: glycohydrolases; pathogenesis-related proteins; proteomics; apoplast proteins; cell wall; bioinformatics
Species: Nicotiana tabacum

Received: July 22, 2010; Accepted: July 13, 2011; Published: September 1, 2012Show citation

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Szabó, E., Szatmári, Á., Hunyadi-Gulyás, É., Besenyei, E., Zsiros, L.R., Bozsó, Z., & Ott, P.G. (2012). Changes in apoplast protein pattern suggest an early role of cell wall structure remodelling in flagellin-triggered basal immunity. Biologia plantarum, 56(3), 551-559. doi: 10.1007/s10535-011-0226-0.
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