Biologia plantarum 57:224-230, 2013 | DOI: 10.1007/s10535-012-0293-x

Transport of mRNA molecules coding NAC domain protein in grafted pear and transgenic tobacco

W. N. Zhang1, X. W. Duan1, C. Ma1, T. Harada2, T. Z. Li1,*
1 Laboratory of Fruit Cell and Molecular Breeding, China Agricultural University, Beijing, P.R. China
2 Laboratory of Plant Breeding and Genetics, Faculty of Agriculture and Life Science, Hirosaki University, Hirosaki, Japan

Grafting is an important cultivation method and recent research on the mechanism of interactions between rootstock nad scion is focused on the long-distance transport of mRNA and small RNAs in the phloem. Among these transportable molecules, NACP gene coding NAM, ATAF1/2, CUC2 (NAC) domain protein might be involved in apical meristem development. Here, we report the transport of NACP mRNA between Chinese pear (Pyrus bretschneideri) cv. Yali (scion) and the wild Pyrus betulaefolia Bunge (rootstock). Our results indicated that NACP mRNA can be transported in both directions from the 3rd to 10th day after micro-grafting. It can also be transported to the shoot apex 30 to 70 cm away from graft-union in 2-year-old grafted trees. For further investigation, transgenic tobaccos with 35S: P. betulaefolia-NACP construct were grafted on wild-type tobaccos (Nicotiana tabacum L. cv. Samsun). The sustainable transport of Pyrus-NACP mRNA through the graft-union occurred from the 15th day after grafting.

Keywords: Pyrus betulaefolia; Pyrus bretschneider; NACP mRNA; Nicotiana tabacum; rootstock; RT-PCR; scion
Subjects: mRNA; NAC domain; grafted tree; transgenic plant; rootstock; RT/PCR; RT-PCR; scion
Species: Nicotiana tabacum; Pyrus betulaefolia; Pyrus bretschneideri

Received: December 26, 2011; Accepted: August 29, 2012; Published: June 1, 2013Show citation

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Zhang, W.N., Duan, X.W., Ma, C., Harada, T., & Li, T.Z. (2013). Transport of mRNA molecules coding NAC domain protein in grafted pear and transgenic tobacco. Biologia plantarum, 57(2), 224-230. doi: 10.1007/s10535-012-0293-x.
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    References

    1. Aida, M., Ishida, T., Fukaki, H., Fujisawa, H., Tasaka, M.: Genes involved in organ separation in Arabidopsis: an analysis of the cup shaped cotyledon mutant. - Plant Cell 9: 841-857, 1997. Go to original source...
    2. Banerjee, A.K., Chatterjee, M., Yu Y., Suh, S.G., Wa M., Hannapel, D.J.: Dynamics of a mobile RNA of potato involved in a long distance signalling pathway. - Plant Cell 18: 3443-3457, 2006. Go to original source...
    3. Banerjee, A.K., Lin, T., Hannapel, D.J.: Untranslated regions of a mobile transcript mediate RNA metabolism. - Plant Physiol. 151: 1831-1843, 2009.
    4. Chang, S., Puryear, J., Cairney, J.: A simple and efficient method for isolating RNA from pine trees. - Plant mol. Biol. Rep. 11: 113-116, 1993. Go to original source...
    5. Clark, S.E.: Cell signalling at the shoot meristem. - Natur. Rev. mol. Cell Biol. 2: 276-284 2001. Go to original source...
    6. Gómez, G., Torres, H., Pallás, V.: Identification of tanslocatable RNA-binding phloem proteins from melon, potential components of the long-distance RNA transport system. - Plant J. 41: 107-116, 2005.
    7. Goodenough, D.A., Goliger, J.A., Paul, D.L.: Connexins, connexons, and intercellular communication. - Annu. Rev. Biochem. 65: 475-502, 1996. Go to original source...
    8. Gurdon, J.B., Dyson, S., Johnson, D.: Cells' perception of position in a concentration gradient. - Cell 95: 159-162, 1998. Go to original source...
    9. Ham, B.K., Brandom, J.L., Xoconostle-Cázares, B., Ringgold, V., Lough, T.J.: A polypyrimidine tract binding protein, pumpkin RBP50, forms the basis of a phloem-mobile ribonucleoprotein complex. - Plant Cell 21: 197-215, 2009. Go to original source...
    10. Haywood, V., Yu, T.S., Huang, N.C., Lucas, W.J.: Phloem long distance trafficking of gibberellic scid-insensitive RNA regulates leaf development. - Plant J. 42: 49-68, 2005. Go to original source...
    11. Huang, N.C., Yu, T.S.: The sequence of Arabidopsis GA sufficient for RNA long-distance trafficking. - Plant J. 59: 921-929, 2009. Go to original source...
    12. Jensen, P.J., Rytter, J., Detwiler, E.A., Travis, J.W., McNellis, T.W.: Rootstock effects on gene expression patterns in apple tree scions. - Plant mol. Biol. 493: 493-511, 2003. Go to original source...
    13. Jensen, P.J., Makalowska, I., Altman, N., Fazio, G., Praul, C., Naximova, S.N., Crassweller, R.M., Travis, J.W., McNellis, T.W.: Rootstock regulated gene expression patterns in apple tree scion. - Tree Genet. Genomes 6: 57-72, 2010. Go to original source...
    14. Kamboj, J.S., Blake, P.S., Quinlan, J.D., Baker, D.A.: Identification and quantitation of GC-MS of zeatin nad zeatin riboside in xylem sap from rootstock and scion of grafted apple trees. - Plant Growth Regul. 28: 199-205, 1999a. Go to original source...
    15. Kamboj, J.S., Browning, G., Blake, P.S., Quinlan, J.D., Baker, D.A.: GC-MS-SIM analysis of abscisic acid and indole-3-acetic acid in shoot bark of apple rootstocks. - Plant Growth Regul. 28: 21-27, 1999b. Go to original source...
    16. Kanehira, A., Yamada, K., Iwaya, T., Tsuwamoto, R., Kasai, A., Nakazono, M., Harada, T.: Apple phloem cells contain some mRNAs transported over long distances. - Tree Genet. Genomes 6: 635-642, 2009.
    17. Kim, M., Canio, W., Kessler, S., Shinha, N.: Developmental changes due to long-distance movement of a homeobox fusion transcript in tomato. - Science 293: 287-289, 2001. Go to original source...
    18. Kragler, F., Yoo, B.C., Lucas, W.J.: RNA as a long-distance information macromolecule in plants. - Mol. Cell Biol. 2: 849-856, 2001.
    19. Kudo, H., Harada, T.: A graft-transmissible RNA from tomato rootstock changes leaf morphology of potato scion. - HortScience 42: 225-226, 2007. Go to original source...
    20. Lucas, W.J., Ding, B., Van der Schoot, C.: Plasmodesmata nad the supracellular nature of plants. - New Phytol. 125: 435-476, 1993. Go to original source...
    21. Lucas, W.J.: Plasmodesmata: intercellular channels for macromolecular transport in plants. - Curr. Opin. Cell Biol. 7: 673-680, 1995. Go to original source...
    22. Mallory, C., Ely, L., Smith, T.H., Marathe, R., Anandalakshmi, R., Fagard, M., Vaucheret, H., Pruss, G., Bowman, L. Vance, V.B.: HC-Pro suppression of transgene silencing eliminates the small RNAs but not transgene methylation or the mobile signal. - Plant Cell 13: 571-583, 2001. Go to original source...
    23. Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures. - Plant Physiol. 15: 473-497, 1962. Go to original source...
    24. Ruiz-Medrano, R., Xoconostle-Cázares, B., Lucas, W.J.: Phloem and long-distance transport of CmNACP mRNA: implications for supracellular regulation in plants. - Development 126: 4405-4419, 1999.
    25. Sablowski, R.W., Meyerowitz, E.M.: A homolog of NO APICAL MERISTEM is an immediate target of the floral homeotic genes APETALA3/PISTILLATA. - Cell 92: 93-103, 1998. Go to original source...
    26. Schmitt, B., Stadler, R., Sauer, N.: Immunolocalization of Solanaceous SUT1 proteins in companion cells and xylem parenchyma: new perspectives for phloem loading nad transport. - Plant Physiol. 148: 187-199, 2008. Go to original source...
    27. Smaka, A., Li, X.Y., Heikelt, C., Welander, M., Zhu, L.H.: Effects of transgenic rootstocks on growth and development of non-transgenic scion cultivars in apple. - Transgenic Res. 19: 933-948, 2010.
    28. Souer, E., Houwelingen, V.A., Kloos, D., Mol, J., Koes, R.: The no apical meristem gene of petunia is required for pattern formation in embryos and flowers and is expressed at meristem boundaries. - Cell 85: 159-170, 1996. Go to original source...
    29. Soumelidou, K., Morris, D.A., Battey, N.H., Barnett, J.R., John, P.: Auxin transport capacity in relation to the dwarfing effect of apple root-stocks. - J. hort. Sci. 69: 719-725, 1994. Go to original source...
    30. Xoconostle-Cázares, B., Xiang, Y., Ruiz-Medrano, R., Wang, H.L., Monzer, J., Yoo, B.C., McFarland, K.C., Franceschi, V.R., Lucas, W.J.: Plant paralog to viral movement protein that potentiates transport of mRNA into the phloem. - Science 283: 94-98, 1999. Go to original source...
    31. Xu, H.Y., Zhang, W.N., Li, M.F., Harada, T., Han, Z.H., Li, T.Z.: Gibberellic acid insensitive mRNA transport in both directions between stock and scion in Malus. - Tree Genet. Genomes 6: 1013-1019, 2010. Go to original source...
    32. Zhang, W.N., Gong, L., Ma, C., Xu, H.Y., Hu, J.F., Li, T.Z.: Gibberellic acid insensitive mRNA transport in Pyrus. - Plant mol. Biol. Rep. 30: 614-623, 2012. Go to original source...

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