Biologia plantarum 2014, 58:296-304 | DOI: 10.1007/s10535-014-0404-y

Long non-coding RNAs in wheat are related to its susceptibility to powdery mildew

S. H. Li1, R. Dudler2, R. Ji3, M. L. Yong1, Z. Y. Wang1, D. W. Hu1,*
1 State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou, P.R. China
2 Institute of Plant Biology, University of Zurich, Zurich, Switzerland
3 State Key Laboratory of Rice Biology, Institute of Insect Science, Zhejiang University, Hangzhou, P.R. China

To identify genes involved in the susceptibility to powdery mildew in wheat, genechip hybridization experiments were performed applying interactions between resistant and susceptible wheat cultivars and powdery mildew. Ten genes were found to express exclusively in the susceptible host after inoculation. The single cell transient expression experiments revealed that the RNA interference (RNAi) of two expressed sequence tag (EST) sequences (accession No. BQ168479 and CA648596) could enhance host resistance by 22 and 15 %, respectively. Full-length cDNA cloning revealed that they represented long non-coding RNAs (lncRNAs) and markedly accumulated during the powdery mildew primary infection.

Keywords: expressed sequence tags; RNA interference; Triticum aestivum
Subjects: non-coding RNAs; pathogen resistance; expressed sequence tags; RNA interference; gene expression; powdery mildew; wheat
Species: Triticum aestivum; Blumeria graminis

Received: August 10, 2013; Revised: November 29, 2013; Accepted: December 19, 2013; Published: June 1, 2014Show citation

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Li, S.H., Dudler, R., Ji, R., Yong, M.L., Wang, Z.Y., & Hu, D.W. (2014). Long non-coding RNAs in wheat are related to its susceptibility to powdery mildew. Biologia plantarum, 58(2), 296-304. doi: 10.1007/s10535-014-0404-y.
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