Biologia plantarum 59:331-340, 2015 | DOI: 10.1007/s10535-015-0492-3

Effect of ochratoxin A and buthionine sulfoximine on proteome and ascorbate-glutathione cycle enzymes in Arabidopsis thaliana

J. R. Hao1, Y. Wang1,4, W. W. Zhao1, W. T. Xu1,2,*, Y. B. Luo1,2, Z. J. Yang2,3, W. H. Wu1,3, Z. H. Liang2, K. L. Huang1,2
1 College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, P.R. China
2 The Supervision, Inspection and Testing Center of Genetically Modified Organisms, Beijing, P.R. China
3 Beijing Key Laboratory of Nutrition, Health and Food Safety, Beijing, P.R. China
4 Institute of Agro-products Processing Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, P.R. China

In this study, proteome and activities of glutathione (GSH)-related enzymes were investigated in detached leaves of Arabidopsis thaliana treated with ochratoxin A (OTA) alone or supplemented with buthionine sulfoximine (BSO, a specific inhibitor of the first step in GSH biosynthesis). A comparative proteomic study using two-dimensional electrophoresis (2-DE) and matrix-assisted laser desorption ionization-time of flight tandem mass spectrometry (MALDI-TOF/TOF MS/MS) identified 12 differentially expressed proteins mainly involved in GSH metabolism, energy metabolism, sugar metabolism, and photosynthesis. The treatment with OTA significantly enhanced the activities of glutathione-S-transferase (GST) and glutathione reductase (GR) through up-regulating the corresponding genes (GSTF7, GR1), an the diminishing effect of BSO on them counteracted the results. However, both OTA and BSO decreased the activity of ascorbate peroxidase (APX), and OTA also decreased the monodehydroascorbate reductase (MDHAR) and glutathione peroxidase (GPX) activities. Briefly, the OTA-induced phytotoxicity to the A. thaliana detached leaves was increased slightly by addition of BSO, and the fluctuation in GSH synthesis, GSH metabolism and disorder of cellular metabolism happened.

Keywords: ascorbate peroxidase; glutathione peroxidase; glutathione reductase; glutathione-S-transferase; monodehydroascorbate reductase
Subjects: ochratoxin A; buthionine sulfoximine; ascorbate-glutathione cycle; proteins; gene expression
Species: Arabidopsis thaliana

Received: January 21, 2014; Revised: August 20, 2014; Accepted: September 23, 2014; Published: June 1, 2015Show citation

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Hao, J.R., Wang, Y., Zhao, W.W., Xu, W.T., Luo, Y.B., Yang, Z.J., ... Huang, K.L. (2015). Effect of ochratoxin A and buthionine sulfoximine on proteome and ascorbate-glutathione cycle enzymes in Arabidopsis thaliana. Biologia plantarum59(2), 331-340. doi: 10.1007/s10535-015-0492-3.
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