Biologia plantarum 50:701-704, 2006 | DOI: 10.1007/s10535-006-0110-5

Improved plant regeneration in Capsicum annuum L. from nodal segments

N. Ahmad1, I. Siddique1, M. Anis1,*
1 Plant Tissue Culture Laboratory, Department of Botany, Aligarh Muslim University, Aligarh, India

Multiple shoots were induced by culturing nodal explants excised from 1-month-old aseptic seedlings of red pepper (Capsicum annuum L. cv. Pusa Jwala) on Murashige and Skoog (MS) medium supplemented with (0.1-10 μM) thidiazuron (TDZ). The rate of multiple shoot induction per explant was maximum (14.4 ± 0.06) on MS medium supplemented with 1.0 μM TDZ. Regenerated shoots were elongated well on growth regulator free MS medium. Adventitious roots were induced two weeks after transfer of elongated shoots to MS medium supplemented with auxins (IAA, IBA or NAA) in different concentrations. Optimum root formation frequency was obtained in medium containing 1.0 μM IBA. Ex-vitro rooting was also achieved by pulse treatment with 300 μM IBA for 10 min. Rooted shoots were transplanted in plastic pots containing garden soil (with 90 % survival rate), where they grew well and attained maturity. Regenerated plants were phenotypically and cytologically normal.

Keywords: axillary shoot; nodal segment; red pepper; thidiazuron
Subjects: auxins; gibberellic acid, gibberellins; in vitro culture, regeneration, proliferation, differentiation; nutrient medium, Murashige and Skoog; red pepper; thidiazuron (TDZ)

Received: September 14, 2004; Accepted: June 2, 2005; Published: December 1, 2006Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
Ahmad, N., Siddique, I., & Anis, M. (2006). Improved plant regeneration in Capsicum annuum L. from nodal segments. Biologia plantarum, 50(4), 701-704. doi: 10.1007/s10535-006-0110-5.
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)

    • Open full article

    References

    1. Agrawal, S., Chandra, N., Kothari, S.L.: Shoot tip culture of pepper for micropropagation.-Curr. Sci. 57: 1347-1349, 1988.
    2. Agrawal, S., Chandra, N., Kothari, S.L.: Plant regeneration in tissue cultures of pepper (Capsicum annuum L. cv. Mathania).-Plant Cell Tissue Organ Cult. 16: 47-55, 1989. Go to original source...
    3. Anis, M.: Cytological investigation in genus Citrus. Inter-and intra-specific variation at meiosis in some species and varieties.-Phytomorphology 48: 349-356, 1998.
    4. Cheepala, S.B., Sharma, N.C., Sahi, S.V.: Rapid in vitro regeneration of Sesbania drummondii.-Biol. Plant. 48: 13-18, 2004. Go to original source...
    5. Christopher, T., Rajam, M.V.: In vitro clonal propagation of Capsicum spp.-Plant Cell Tissue Organ Cult. 38: 25-29, 1994. Go to original source...
    6. Çöçü, S., Uranbey, S., İpek, A., Khawar, K.M., Sarihan, E.O., Kaya, M.D., Parmaksiz, İ., Özcan, S.: Adventitious shoot regeneration and micropropagation in Calendula officinalis L.-Biol. Plant. 48: 449-451, 2004.
    7. Dolendro, S.N., Sahoo, L., Bhalla, S.N., Jaiwal, P.K.: The effect of TDZ on organogenesis and somatic embryogenesis in pigeon pea (Cajanus cajan L. Mill sp).-Plant Sci. 164: 341-347, 2003.
    8. Franck-Duchenne, M., Wang, Y., Tahar, S.F., Beachy, R.N.: In vitro stem elongation of sweet pepper in media containing 24-epi brassinolide.-Plant Cell Tissue Organ Cult. 53: 79-84, 1998. Go to original source...
    9. Hyde, C., Phillips, G.C.: Silver nitrate promotes plant regeneration of Chile pepper (Capsicum annuum L.) via organogenesis.-In Vitro cell. dev. Biol. Plant 32: 72-80, 1996. Go to original source...
    10. Kintzios, S., Drossopoulos, J.B., Shortsianitis, E., Peppes, D.: Induction of somatic embryogenesis from young fully expanded leaves of chilli pepper (Capsicum annuum L.): effect of leaf position, illumination and explant pretreatment with high cytokinin concentrations.-Scientia Hort. 85: 137-144, 2000. Go to original source...
    11. Malabadi, R.B., Mulgund, G.S., Natraja, K.: Efficient regeneration of Vanda coerulea, an endangered orchid using thidiazuron.-Plant Cell Tissue Organ Cult. 76: 289-293, 2004. Go to original source...
    12. Manoharan, M., Vidya, C.S.S., Sita, G.L.: Agrobacterium mediated genetic transformation in hot chilli (Capsicum annuum L var. Pusa Jwala).-Plant Sci. 131: 77-83, 1998. Go to original source...
    13. Murashige, T., Skoog, F. A revised medium for rapid growth and bioassays with tobacco tissue cultures.-Physiol Plant 15: 473-497, 1962. Go to original source...
    14. Ochoa-Alejo, N., Ramirez-Malagon, R.: In vitro chilli pepper Biotechnology.-In Vitro cell. dev. Biol. Plant 37: 701-729, 2001.
    15. Steinitz, B., Wolf, D., Matzevitch-Josef, T., Zelcer, A.: Regeneration in vitro and genetic transformation of pepper (Capsicum spp.). The current state of art.-Capsicum and Egg Plant Newslett. 18: 9-15, 1999.
    16. Valera-Montero, L.L., Ochoa Alejo, N.: A novel approach for chili pepper (Capsicum annuum L.) plant regeneration: Shoot induction in rooted hypocotyls.-Plant Sci. 84: 215-219, 1992. Go to original source...
    17. Venkataiah, P., Christopher, T., Subhash, K.: Thidiazuron induced high frequency adventitious shoot formation and plant regeneration in Capsicum annuum L.-Plant Biotechnol. 5: 245-25, 2003.

    Return to the content