Biologia plantarum 52:141-145, 2008 | DOI: 10.1007/s10535-008-0029-0

In vitro plant regeneration in six cultivars of Capsicum spp. using different explants

K. Sanatombi1, G. J. Sharma1,*
1 Department of Life Sciences, Manipur University, Imphal, India

In vitro regeneration from leaf, cotyledon and hypocotyl explants of six cultivars belonging to three species of Capsicum was achieved by direct organogenesis. The cultivar Umorok showed the best response while Meiteimorok, Haomorok, Mashingkha and Uchithi showed intermediate response and the cultivar Chiengpi was the least responsive. Leaf and cotyledon explants regenerated more shoots than hypocotyl explants and the maximum number of shoots were produced on Murashige and Skoog (1962) medium containing 8.8 µM 6-benzylaminopurine (BAP) with 11.4 µM indole-3-acetic acid (IAA). Elongation of shoot buds derived from different explants was achieved on medium containing 2.8 µM IAA and the elongated shoots were rooted on medium containing 2.8 or 5.7 µM IAA and 2.4 or 4.9 µM indole-3-butyric acid (IBA). Four-week old rooted plantlets were hardened and transplanted to the soil. The plantlets showed 90 % survival during transplantation.

Keywords: bud induction; Capsicum annuum; C. chinense; C. frutescens; direct organogenesis; micropropagation
Subjects: auxins; Capsicum annuum; Capsicum chinense; Capsicum frutescens; cotyledon, cotyledonary node; in vitro culture, micropropagation; in vitro culture, rooting; nutrient medium, Murashige and Skoog (MS)

Received: November 30, 2006; Accepted: November 17, 2007; Published: March 1, 2008Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
Sanatombi, K., & Sharma, G.J. (2008). In vitro plant regeneration in six cultivars of Capsicum spp. using different explants. Biologia plantarum, 52(1), 141-145. doi: 10.1007/s10535-008-0029-0.
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Agrawal, S., Chandra, N., Kothari, S.L.: Plant regeneration in tissue cultures of pepper (Capsicum annuum L. cv. Mathania).-Plant Cell Tissue Organ Cult. 16: 47-55, 1989. Go to original source...
    2. Arroyo, R., Revilla, M.A.: In vitro plant regeneration from cotyledon and hypocotyl segments in two bell pepper cultivars.-Plant Cell Rep. 10: 414-416, 1991. Go to original source...
    3. Carputo, D., Cardi, T., Chiari, T., Ferraiolo, G., Frusciante, L.: Tissue culture response in various wild and cultivated Solanum germplasm accessions for exploitation in potato breeding.-Plant Cell Tissue Organ Cult. 41: 151-158, 1995. Go to original source...
    4. Christopher, T., Rajam, M.V.: In vitro clonal propagation of Capsicum spp.-Plant Cell Tissue Organ Cult. 38: 25-29, 1994. Go to original source...
    5. Christopher, T., Rajam, M.V.: Effect of genotype, explants and medium on in vitro regeneration of red pepper.-Plant Cell Tissue Organ Cult. 46: 245-250, 1996. Go to original source...
    6. Ebida, A.I.A., Hu, C.Y.: In vitro morphogenetic responses and plant regeneration from pepper (Capsicum annuum L. cv. 'California Wonder) seedling explants.-Plant Cell Rep. 13: 107-110, 1993. Go to original source...
    7. Ezura, H., Nishimiya, S., Kasumi, M.: Efficient regeneration of plants independent of exogenous growth regulators in bell pepper (Capsicum annuum L.).-Plant Cell Rep. 12: 676-680, 1993. Go to original source...
    8. Frank-Duchenne, M., Wang, Y., Tahar, S.B., Beachy, R.N.: In vitro stem elongation of sweet pepper in media containing 24-epi-brassinolide.-Plant Cell Tissue Organ Cult. 53: 79-84, 1998. Go to original source...
    9. Gunay, A.L., Rao, P.S.: In vitro plant regeneration from hypocotyl and cotyledon explants of red pepper (Capsicum).-Plant Sci. Lett. 11: 365-372, 1978. Go to original source...
    10. Husain, S., Jain, A., Kothari, S.L.: Phenylacetic acid improves bud elongation and in vitro plant regeneration efficiency in Capsicum annuum L.-Plant Cell Rep. 19: 64-68, 1999. Go to original source...
    11. Hyde, C.L., Phillips, G.C.: Silver nitrate promotes shoot development and plant regeneration of chilli pepper (Capsicum annuum L.) via organogenesis.-In Vitro cell dev. Biol. Plant. 32: 72-80, 1996.
    12. Khan, H., Siddique, I., Anis, M.: Thidiazuron induced somatic embryogenesis and plant regeneration in Capsicum annuum.-Biol. Plant. 50: 789-792, 2006. Go to original source...
    13. Liu, W., Parrot, W.A., Hildebrand, D.F., Collins, G.B.: Williams, E.G.: Agrobacterium induced gall formation in bell pepper (Capsicum annuum L.) and formation of shoot-like structures expressing introduced genes.-Plant Cell Rep. 9: 360-364, 1990. Go to original source...
    14. M'Ribu, H.K., Veilleux, R.E.: Effect of genotype, explant, subculture interval and environmental conditions on regeneration of shoots from in vitro monoploids of a diploid potato species, Solanum phureja Juz. & Buk.-Plant Cell Tissue Organ Cult. 23: 171-179, 1990.
    15. Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures.-Physiol. Plant. 15: 473-497, 1962. Go to original source...
    16. Ochoa-Alejo, N., Ireta-Moreno, L.: Cultivar differences in shoot-forming capacity of hypocotyl tissues of chilli pepper (Capsicum annuum L.) cultured in vitro.-Scientia Hort. 42: 21-28, 1990. Go to original source...
    17. Phillips, G.C., Hubstenberger, J.F.: Organogenesis in pepper tissue cultures.-Plant Cell Tissue Organ Cult. 4: 261-269, 1985. Go to original source...
    18. Ramage, C.M., Leung, W.M.: Influence of BA and sucrose on the competence and determination of pepper (Capsicum annuum L. var. Sweet Banana) hypocotyl cultures during shoot formation.-Plant Cell Rep. 15: 974-979, 1996. Go to original source...
    19. Ramirez-Malagon, R., Ochoa-Alejo, N.: An improved and reliable chilli pepper (Capsicum annuum L.) plant regeneration method.-Plant Cell Rep. 16: 226-231, 1996. Go to original source...
    20. Sanatombi, K., Sharma, G.J.: Micropropagation of Capsicum frutescens L. using axillary shoot explants.-Scientia Hort. 113: 96-99, 2007. Go to original source...
    21. Sanatombi, K., Sharma, G.J.: In vitro propagation of Capsicum frutescens L. cv. Umorok, the hottest chilli cultivar.-Biol. Plant. 2008 (in press). Go to original source...
    22. Sharma, P., Rajam, M.V.: Genotype, explant, and position effects on organogenesis and embryogenesis in eggplant (Solanum melongena).-J. exp. Bot. 46: 135-141, 1995. Go to original source...
    23. Sripichitt, P., Nawata, E., Shigenaga, S.: In vitro shoot forming capacity of cotyledon explants in red pepper (Capsicum annuum L. cv 'Yatsufusa).-Jap. J. Breed. 37: 133-142, 1987. Go to original source...
    24. Steinitz, B., Wolf, D., Matzevitch-Josef, T., Zelcar, A.: Regeneration in vitro and genetic transformation of pepper (Capsicum spp.): The current state of art.-Capsicum Eggplant News Lett. 18: 9-15, 1999.
    25. Szasz, A., Nervo, G., Fari, M.: Screening for in vitro shoot forming capacity of seedling explants in bell pepper (Capsicum annuum L.) genotypes and efficient plant regeneration using thidiazuron.-Plant Cell Rep. 14: 666-669, 1995. Go to original source...
    26. Venkataiah, P., Christopher, T., Subhash, K.: Thidiazuron induced high frequency adventitious shoot formation and plant regeneration in Capsicum annuum L.-J. Plant Biotechnol. 5: 245-250, 2003.

    Return to the content