Biologia plantarum 54:154-158, 2010 | DOI: 10.1007/s10535-010-0025-z

Characterization of a xylanase inhibitor TAXI-I from wheat

X. -Y. Weng1, Y. -Y. Huang1, H. Gao2, J. -Y. Sun2,*
1 College of Life Science, Zhejiang University, Hangzhou, P.R.China
2 College of Animal Science, Zhejiang University, Hangzhou, P.R.China

Xylanase inhibitor TAXI-I gene was cloned from wheat (Triticum aestivum L.) and then TAXI-I encoding sequence was expressed in Escherichia coli. The recombinant TAXI-I protein inhibited glycoside hydrolase (GH) family 11 xylanases in Aspergillus niger (Anx; a fungal xylanase), and Thermomonospora fusca (Tfx; a bacterial xylanase), and also inhibited hybrid xylanases Atx (a hybrid xylanase whose parents are T. fusca and A. niger) and Btx (a hybrid xylanase whose parents are T. fusca and Bacillus subtilis). Among the tested xylanases, A. niger xylanase was the most inhibited one by wheat xylanase inhibitor TAXI-I, while T. fusca xylanase was the least inhibited one. The profile of TAXI-I gene expression in wheat in response to phytohormones was also investigated. TAXI-I gene expression was drastically induced by methyl jasmonate (MeJa), and hardly detected in gibberellic acid (GA) treatment. Therefore, TAXI-I might be involved in plant defense against fungal and bacteria xylanases.

Keywords: Aspergillus niger; Bacillus subtilis; plant defense; stress response; Triticum aestivum
Subjects: amino acid sequences; Aspergillus niger; Bacillus subtilis; Escherichia coli; gibberellic acid, gibberellin; jasmonates; nucleotide sequences; Thermomonospora fusca; Triticum aestivum; wheat; xylanase inhibitor

Received: May 6, 2008; Accepted: November 1, 2008; Published: March 1, 2010Show citation

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Weng, X.-Y., Huang, Y.-Y., Gao, H., & Sun, J.-Y. (2010). Characterization of a xylanase inhibitor TAXI-I from wheat. Biologia plantarum, 54(1), 154-158. doi: 10.1007/s10535-010-0025-z.
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