Biologia plantarum 49:475-479, 2005 | DOI: 10.1007/s10535-005-0034-5

Sucrose accumulation and enzyme activities in callus culture of sugarcane

F. A. Gutierrez-Miceli1, M. A. Rodriguez-Mendiola1, N. Ochoa-Alejo1, R. Mendez-Salas1, C. Arias-Castro1, L. Dendooven1,*
1 Departamento de Biotecnologia y Bioingenieria, CINVESTAV-IPN, Mexico DF, Mexico

The activities of sucrose phosphate synthase (SPS), sucrose synthase (SUSY), neutral invertase (NI) and soluble acid invertase (SAI) were measured in callus cultures of four Mexican sugarcane cultivars (Saccharum spp.) with a different capacity to accumulate sucrose in stem parenchyma cells. The results indicated that sucrose accumulation in callus was positively correlated to the activity of SPS and SUSY and negatively to the activity of SAI and NI while SPS explained most of the variation found for sucrose accumulation and NI least.

Keywords: neutral invertase; Saccharum spp.; soluble acid invertase; sucrose phosphate synthase; sucrose synthase
Subjects: fructokinase; glucokinase; glucose-6-phosphate dehydrogenase; Nicotiana tabacum; phosphodiesterase; 6-phosphogluconate dehydrogenase; phosphomonoesterase; potato virus Y; protein, PVY and TMV infected tobacco; ribonuclease; soluble acid invertase; sucrose-phosphate synthase; sucrose synthase; sugar cane, regeneration, sucrose accumulation; tobacco, mosaic virus; tobacco, PVY and TMV infected; viral-RNA biosynthesis; virus

Received: July 5, 2003; Accepted: December 2, 2004; Published: September 1, 2005Show citation

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Gutierrez-Miceli, F.A., Rodriguez-Mendiola, M.A., Ochoa-Alejo, N., Mendez-Salas, R., Arias-Castro, C., & Dendooven, L. (2005). Sucrose accumulation and enzyme activities in callus culture of sugarcane. Biologia plantarum, 49(3), 475-479. doi: 10.1007/s10535-005-0034-5.
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