Biologia plantarum 51:551-555, 2007 | DOI: 10.1007/s10535-007-0119-4

Application of fluorimetric analysis of plant esterases to study of programmed cell death and effects of cadmium(II) ions

J. Víteček1, J. Petrlová2, J. Petřek1, V. Adam2,3, L. Havel1, K. J. Kramer4, R. Kizek2,*
1 Department of Plant Biology, Mendel University of Agriculture and Forestry, Brno, Czech Republic
2 Department of Chemistry and Biochemistry, Mendel University of Agriculture and Forestry, Brno, Czech Republic
3 Department of Analytical Chemistry, Faculty of Science, Masaryk University, Brno, Czech Republic
4 Grain Marketing and Production Research Center, Agricultural Research Service, US Department of Agriculture, Manhattan, USA

Esterases (EC 3.1.1.x) represent a diverse group of hydrolases catalyzing the cleavage and formation of carboxyl ester bonds. Their connection with development has made them a suitable marker of development in plants. In the present work, we focused on the fluorimetric determination of the plant esterases in plant cell cultures (tobacco BY-2 cells and early somatic embryos of Norway spruce, clone 2/32) with respect to application the method for the study of programmed cell death and the influence of cadmium(II) ions on the plant cells. The programmed cell death has been triggered by sodium nitroprusside and glucose oxidase. The determination of the esterase activity by the proposed technique in a cell extract determined very small difference in enzyme activity, which was a reliable marker of metabolic changes. In addition, the esterase activity of spruce somatic embryos decreased with the increase in medium Cd concentration.

Keywords: attomole detection; confocal microscopy; early somatic embryos of Norway spruce; fluorescein diacetate; fluorescence microscopy; protoplast; tobacco BY-2
Subjects: cadmium; cell death, programmed; esterases; fluorescein diacetate; in vitro culture, embryogenesis, somatic embryogenesis; Norway spruce; protoplasts; sodium nitroprusside; tobacco

Received: December 15, 2005; Accepted: November 11, 2006; Published: September 1, 2007Show citation

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Víteček, J., Petrlová, J., Petřek, J., Adam, V., Havel, L., Kramer, K.J., & Kizek, R. (2007). Application of fluorimetric analysis of plant esterases to study of programmed cell death and effects of cadmium(II) ions. Biologia plantarum, 51(3), 551-555. doi: 10.1007/s10535-007-0119-4.
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    References

    1. Ahearn, G.A., Mandal, P.K., Mandal, A.: Mechanisms of heavy-metal sequestration and detoxification in crustaceans: a review.-J. comp. Physiol. B 174: 439-452, 2004. Go to original source...
    2. Chen, Y.H., Li, X.D., Shen, Z.G.: Leaching and uptake of heavy metals by ten different species of plants during an EDTA-assisted phytoextraction process.-Chemosphere 57: 187-196, 2004. Go to original source...
    3. Cummins, I., Burnet, M., Edwards, R.: Biochemical characterisation of esterases active in hydrolysing xenobiotics in wheat and competing weeds.-Physiol. Plant. 113: 477-485, 2001. Go to original source...
    4. Delledonne, M., Murgia, I., Ederle, D., Sbicego, P.F., Biondani, A., Polverari, A., Lamb, C.: Reactive oxygen intermediates modulate nitric oxide signaling in the plant hypersensitive disease-resistance response.-Plant Physiol. Biochem. 40: 605-610, 2002. Go to original source...
    5. De Pinto, M.C., Tommasi, F., De Gara, L.: Changes in antioxidant systems as part of the signalling pathway responsible for the programmed cell death activated by nitric oxide and reactive oxygen species in tabacco Bright-Yellow 2 cells.-Plant Physiol. 130: 698-708, 2002. Go to original source...
    6. Durzan, D.J., Jokinen, K., Guerra, M.P., Santerre, A., Chalupa, V., Havel, L.: Latent diploid parthenogenesis and parthenote cleavage in egg-equivalents of Norway spruce.-Int. J. Plant Sci. 155: 677-688, 1994. Go to original source...
    7. Gupta, D.K., Tohoyama, H., Joho, M., Inouhe, M.: Changes in the levels of phytochelatins and related metal-binding peptides in chickpea seedlings exposed to arsenic and different heavy metal ions.-J. Plant Res. 117: 253-256, 2004. Go to original source...
    8. Havel, L., Durzan, D.J.: Apoptosis during diploid parthenogenesis and early somatic embryogenesis of Norway spruce.-Int. J. Plant Sci. 157: 8-16, 1996a. Go to original source...
    9. Havel, L., Durzan, D.J.: Apoptosis in plants.-Bot. Acta 109: 268-277, 1996b. Go to original source...
    10. Jokinen, K.J., Durzan, D.J.: Properties of rescued embryonal suspensor mass of Norway spruce (Picea abies L. Karst.) determined by the genotype and environment in vitro.-Silvae fenn. 28: 95-106, 1994. Go to original source...
    11. Jones, K.H., Senft, J.A.: An improved method to determine cell viability by simultaneous staining with fluorescein diacetate propidium iodide.-J. Histochem. Cytochem. 33: 77-79, 1985. Go to original source...
    12. Kizek, R., Trnková, L., Ševčíková, S., Šmarda, J., Jelen, F.: Silver electrode as a sensor for determination of zinc in cell cultivation medium.-Anal. Biochem. 301: 8-13, 2002. Go to original source...
    13. Klejdus, B., Zehnálek, J., Adam, V., Petřek, J., Kizek, R., Vacek, J., Trnková, L., Rozik, R., Havel, L., Kubáň, V.: Sub-picomole high-performance liquid chromatographic/ mass spectrometric determination of glutathione in the maize (Zea mays L.) kernels exposed to cadmium.-Anal. chim. Acta 520: 117-124, 2004. Go to original source...
    14. Krulíčková, K., Pošvec, Z., Griga, M.: Identification of flax and linseed cultivars by isozyme markers.-Biol. Plant. 45: 327-336, 2002. Go to original source...
    15. Mlejnek, P., Procházka, S.: Activation of caspase-like proteases and induction of apoptosis by isopentenyladenosine in tobacco BY-2 cells.-Planta 215: 158-166, 2002. Go to original source...
    16. Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures.-Physiol. Plant. 15: 473, 1962. Go to original source...
    17. Nagata, T., Nemoto, Y., Hasezawa, S.: Tobacco BY-2 cell line as the "HeLa" cell line in the cell biology of higher plants.-Int. Rev. Cytol. 132: 1-30, 1992. Go to original source...
    18. Neill, S.J., Desikan, R., Clarke, A., Hurst, R.D., Hancock, J.T.: Hydrogen peroxide and nitric oxide as sigalling molecules in plants.-J. exp. Bot. 53: 1237-1247, 2002. Go to original source...
    19. Österås, A.H., Greger, M.: Interactions between calcium and copper or cadmium in Norway spruce.-Biol. Plant. 50: 647-652, 2006. Go to original source...
    20. Petřek, J., Víteček, J., Vlašínová, H., Kizek, R., Kramer, K.J., Adam, V., Klejdus, B., Havel, L.: Application of computer imaging, stripping voltammetry and mass spectrometry for study of the effect of lead (Pb-EDTA) on growth and viability of early somatic embryos of Norway spruce (Picea abies /L./ Karst.).-Anal. bioanal. Chem. 383: 576-586, 2005.
    21. Potěšil, D., Petrlová, J., Adam, V., Vacek, J., Klejdus, B., Zehnálek, J., Trnková, L., Havel, L., Kizek, R.: Simultaneous femtomole determination of cysteine, reduced and oxidized glutathione, and phytochelatin in maize (Zea mays L.) kernels using high-performance liquid chromatography with electrochemical detection.-J. Chrom. A 1084: 134-144, 2005.
    22. Qadir, S., Qureshi, M.I., Javed, S., Abdin, M.Z.: Genotypic variation in phytoremediation potential of Brassica juncea cultivars exposed to Cd stress.-Plant Sci. 167: 1171-1181, 2004. Go to original source...
    23. Sandermann, H.: Plant metabolism of xenobiotics.-Trends Biochem. Sci. 17: 82-84, 1992. Go to original source...
    24. Stoilova, T., Cholakova, N., Markova, M.: Variation in seed protein and isoenzyme patterns in Cucurbita cultivars.-Biol. Plant. 50: 450-452, 2006. Go to original source...
    25. Stuhlfelder, C., Lottspeich, F., Mueller, M.J.: Purification and partial amino acid sequences of an esterase from tomato.-Phytochemistry 60: 233-240, 2002. Go to original source...
    26. Suresh, B., Ravishankar, G.A.: Phytoremediation-A novel and promising approach for environmental clean-up.-Crit. Rev. Biotechnol. 24: 97-124, 2004. Go to original source...
    27. Tsanev, V., Vladova, R., Petkolicheva, K., Kraptchev, B., Milanov, C.: Esterase isoenzymes as markers for the VA 1 gene of Zea mays and for the B linkage group of Tripsacum dactyloides.-Biol. Plant. 48: 437-439, 2004. Go to original source...
    28. Vacek, J., Petřek, J., Kizek, R., Havel, L., Klejdus, B., Trnková, L., Jelen, F.: Electrochemical determination of lead and glutathione in a plant cell culture.-Bioelectrochemistry 63: 347-351, 2003.
    29. Víteček, J., Adam, V., Petřek, J., Babula, P., Novotná, P., Kizek, R., Havel, L.: [Application of fluorimetric determination of esterases in plant material]-Chem. Listy 99: 496-501. 2005. [In Czech.]
    30. Víteček, J., Adam, V., Petřek, J., Vacek, J., Kizek, R., Havel, L.: Esterases as a marker for growth of BY-2 tobacco cells and early somatic embryos of the Norway spruce.-Plant Cell Tissue Organ Cult. 79: 195-201, 2004.
    31. Von Arnold, S.J.: Improved efficiency of somatic embryogenesis in mature embryos of Picea abies (L.).-Plant Physiol. 127: 233-244, 1987. Go to original source...
    32. Willats, W.G.T., Orfila, C., Limberg, G., Buchholt, H.C., Van Alebeek, G.-J.W.M., Voragen, A.G.J., Marcus, S.E., Christensen, T.M.I.E., Mikkelsen, J.D., Murray, B.S., Knox, J.P.: Modulation of the degree and pattern of methylesterification of pectic homogalacturonan in plant cell walls.-J. biol. Chem. 276: 19404-19413, 2001. Go to original source...
    33. Wojcik, M., Tukiendorf, A.: Cadmium uptake, localization and detoxification in Zea mays.-Biol. Plant. 49: 237-245, 2005. Go to original source...
    34. Zehnálek, J., Adam, V., Kizek, R.: Application of higher plants in phytoremetiation of heavy metals.-Listy Cukrov. 120: 222-224 2004.

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