Biologia plantarum 52:727-731, 2008 | DOI: 10.1007/s10535-008-0140-2

Antioxidative response to cadmium in roots and leaves of tomato plants

W. B. Ammar1,*, I. Nouairi2, M. Zarrouk2, M. H. Ghorbel1, F. Jemal1
1 Unité de Recherche Nutrition et Métabolisme Azotés et Protéines de Stress, Faculté des Sciences de Tunis, Campus Universitaire, Tunis El Manar, Tunisie
2 Laboratoire Caractérisation et Qualité de l'Huile d'Olive, Centre de Biotechnologie de Borj-Cedria B, P.901, Hammam-Lif, Tunisia

Treatment of tomato seedlings (Lycopersicon esculentum Mill. cv. 63/5 F1) with increasing CdCl2 concentrations in the culture medium resulted in Cd accumulation more important in roots than in leaves. Biomass production was severely inhibited, even at low Cd concentration. Cd reduced chlorophyll content in leaves and enhanced lipid peroxidation. An increase in antioxidative enzyme (superoxide dismutase, ascorbate peroxidase, guaiacol peroxidase, glutathione reductase) activities was more pronounced in leaves than in roots, while catalase activity increased only in roots. In addition, changes in isoenzyme composition were observed using the non-denaturing polyacrylamid gel electrophoresis.

Keywords: lipid peroxidation; Lycopersicon esculentum; oxidative stress
Subjects: antioxidants, antioxidant enzymes; ascorbate peroxidase; carotenoids; catalase; chlorophyll a,b; glutathione reductase; guaiacol peroxidase; heavy metals; lipid peroxidation; Lycopersicon esculentum; peroxidase; superoxide dismutase (SOD); tomato

Received: November 22, 2006; Accepted: September 11, 2007; Published: December 1, 2008Show citation

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Ammar, W.B., Nouairi, I., Zarrouk, M., Ghorbel, M.H., & Jemal, F. (2008). Antioxidative response to cadmium in roots and leaves of tomato plants. Biologia plantarum, 52(4), 727-731. doi: 10.1007/s10535-008-0140-2.
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    References

    1. Agrawal, V., Sharma, K.: Phytotoxic effects of Cu, Zn, Cd and Pb on in vitro regeneration and concomitant protein changes in Holarrhena antidysenterica.-Biol. Plant. 50: 307-310, 2006. Go to original source...
    2. Alia, K.V.S.K., Prasad, P., Pardha Saradhi, P.: Effect of zinc on free radicals and proline in Brassica and Cajanus.-Phytochemistry 42: 45-47, 1995. Go to original source...
    3. Allen, C., Good, P.: Acyl lipid in photosynthetic systems.-Methods Enzymol. 23: 523-547, 1971. Go to original source...
    4. Alsher, R.G., Erturk, N., Heath, L.S.: Role of superoxide dismutases (SODs) in controlling oxidative stress.-J. exp. Bot. 53: 1331-1341, 2002. Go to original source...
    5. Anderson, M.E.: Determination of glutathione and glutathione disulfide in biological samples. Methods Enzymol. 113: 548-555, 1985. Go to original source...
    6. Arnon, D.I.: Copper enzymes in isolated chloroplast polyphenoloxydases in Beta vulgaris.-Plant Physiol. 24: 1-15, 1949. Go to original source...
    7. Ben Ammar, W., Nouairi, I., Tray, B., Zarrouk, M., Jemal, F., Ghorbal, M.H.: Effets du cadmium sur l'accumulation ionique et les teneurs en lipides dans les feuilles de tomate (Lycopersicon esculentum).-J. Soc. Biol. (France) 199: 157-163, 2005. Go to original source...
    8. Ben Youssef, N., Nouairi, I., Ben Temime, S., Taamalli, W., Zarrouk, M., Ghorbal, M.H., Ben Miled Daoud, D.: Effets du cadmium sur le métabolisme des lipides de plantules de colza (Brassica napus L.).-Compt. rend. Biol. 328: 745-757, 2005. Go to original source...
    9. Beyer, W.F., Fridivich, Y.: Assaying for superoxide dismutase activity: some large consequences of minor changes in conditions.-Ann. Biochem. 161: 559-566, 1987. Go to original source...
    10. Bradford, M.M.: A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding.-Ann. Biochem. 72: 248-254, 1976. Go to original source...
    11. Chaparro-Giraldo, A., Barata, R.M., Chabregas, S.M., Azevedo, R.A., Silva-Filho, M.C.: Soybean leghemoglobilin targeted to potato chloroplasts influences growth and development of transgenic plants.-Plant Cell Rep. 19: 961-965, 2000. Go to original source...
    12. Chen, G.X., Asada, K.: Ascorbate peroxidase in tea leaves: Occurrence of two isozymes and the differences in their enzymatic and molecular properties.-Plant Cell Physiol. 30: 987-998, 1989.
    13. Cho, U., Seo, N.: Oxidative stress in Arabidopsis thaliana exposed to cadmium is due to hydrogen peroxide accumulation.-Plant Sci. 168: 113-120, 2005. Go to original source...
    14. Clemens, S.: Molecular mechanisms of plant metal tolerance and homeostasis.-Plant. 212: 475-486, 2001. Go to original source...
    15. Cobbett, C.S.: Phytochelatins and their roles in heavy metal detoxification.-Plant Physiol. 123: 825-832, 2000. Go to original source...
    16. De Pinto, M.C., Tommasi, F., De Gara, L.: Enzymes of the ascorbate biosynthesis and ascorbate-glutathione cycle in cultured cells of tobacco bright yellow (BY-2 line).-Plant Physiol. Biochem. 38: 541-550, 2000. Go to original source...
    17. Dixit, V., Pandey, V., Shyam, R.: Differential antioxidative responses to cadmium in roots and leaves of pea (Pisum sativum L. cv. Azad).-J. exp. Bot. 52: 1101-1109, 2001. Go to original source...
    18. Drazić, G., Mihailović, N., Lojić, M.: Cadmium accumulation in Medicago sativa seedlings treated with salicylic acid.-Biol. Plant. 50: 239-244, 2006. Go to original source...
    19. Ferreira, R.R., Fornazier, R.F., Vitória, A.P., Molina, S.M.G., Lea, P.J., Azevedo, R.A.: Changes in antioxidant enzyme activities in soybean under cadmium stress.-J. Plant. Nutr. 25: 327-342, 2002. Go to original source...
    20. Fink, R.C., Scandalios, J.G.: Molecular evolution and structure-function relationships of the superoxide dismutase gene families in angiosperms and their relationship to other eukaryotic and prokaryotic superoxide dismutases.-Arch. Biochem. Biophys. 399: 19-36, 2002. Go to original source...
    21. Inhnoue, M.: Phytochelatins.-Braz. J. Plant physiol. 17: 65-78, 2005. Go to original source...
    22. Kopyra, M., Gwóźdź, E.A.: Antioxidant enzymes in parquet and cadmium resistant cell lines of horseradish.-Biol. Lett. 40: 61-69, 2003.
    23. Landberg, T., Greger, M.: Differences in oxidative stress in heavy metal resistant and sensitive clones of Salix viminalis.-J. Plant Physiol. 159: 69-75, 2002. Go to original source...
    24. León, A.M., Palma, J.M., Corpas, F.J., Gómez, M., Romero-Puertas, M.C., Chatterjee, D., Mateos, R.M., Del Pietrini, F., Iannelli, M.A., Pasqualini, S., Massacci, A.: Interaction of cadmium with glutathione and photosynthesis in developing leaves and chloroplasts of Phragmites australis.-Plant Physiol. 133: 829-837, 2003.
    25. Mittal, R., Dubey, R.S.: Behaviour of peroxidases in rice: changes in enzyme activity and isoforms in relation to salt tolerance.-Plant. Physiol. Biochem. 29: 31-40, 1991.
    26. Mittler, R.: Oxidative stress, antioxidants and stress tolerance.-Trends Plant. Sci. 7: 405-410, 2002. Go to original source...
    27. Pietrini, F., Iannelli, M.A., Pasqualini, S., Massacci, A.: Interaction of cadmium with glutathione and photosynthesis in developing leaves and chloroplasts of Phragmites australis.-Plant Physiol. 133: 829-837, 2003. Go to original source...
    28. Pereira, G.J.G., Molina, S.M.G., Lea, P.J., Azevedo, R.A.: Activity of antioxidant enzymes in response to cadmium in Cortalaria juncea.-Plant. Soil. 239: 123-132, 2002. Go to original source...
    29. Pinto, E., Sigaud-Kutner, T.C.S., Leita, M.A.S., Okamoto, O.K., Morse, D., Colepicolo, P.: Heavy metal-induce oxidative stress in algae.-J. Phycol. 39: 1008-1018, 2003. Go to original source...
    30. Rao, M.V., Paliyath, G., Ormrod, D.P.: Ultraviolet-B-and ozone-induced biochemical changes in antioxidant enzymes of Arabidopsis thaliana.-Plant Physiol. 110: 125-36, 1996. Go to original source...
    31. Rio, L.A., Sandalio, L.M.: Antioxidative enzymes in cultivars of pepper plants with different sensitivity to cadmium.-Plant. Physiol. Biochem. 40: 813-820, 2002.
    32. Scebba, F., Arduini, I., Ercoli, L., Sebastiani, L.: Cadmium effects on growth and antioxidant enzymes activities in Miscanthus sinensis.-Biol. Plant. 50: 688-692, 2006. Go to original source...
    33. Schickler, H., Caspi, H.: Response of antioxidative enzymes to nickel and cadmium stress in hyperaccumulator plants of the genus Alyssum.-Physiol. Plant. 105: 39-44, 1999. Go to original source...
    34. Yu, C.W., Murphy, T.M., Sung, W.W., Lin, C.H.: H2O2 treatment induces glutathione accumulation and chilling tolerance in mung bean.-Funct. Plant Biol. 29: 1081-1087, 2000.

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