Biologia plantarum 57:769-772, 2013 | DOI: 10.1007/s10535-013-0341-1

Effect of irradiance during acclimatization on content of proline and phytohormones in micropropagated Ulmus minor

M. C. Dias1,*, G. Pinto1, C. Guerra1, C. Jesus1, J. Amaral1, C. Santos1
1 Department of Biology and Centre for Environmental and Marine Studies, University of Aveiro, Aveiro, Portugal

This study aimed to investigate the effects of irradiance on plant growth and content of proline and phytohormones during ex vitro acclimatization of micropropagated Ulmus minor plants. In vitro rooted plants were acclimatized to ex vitro conditions in a climate chamber with two irradiances, 200 μmol m-2 s-1 (high irradiance, HI) and 100 μmol m-2 s-1 (low irradiance, LI) for 40 d. Immediately after the ex vitro transfer, the plants experienced a water deficit [wilting leaves with the reduced relative water content (RWC)], but following the experiment, the recovery of the RWC was more pronounced in the HI treatment. Also, the content of proline, ABA, and JA-Ile were higher in HI treatment. Growth analyses revealed that HI improved growth and biomass production.

Keywords: abscisic acid; growth analysis; jasmonic acid; net assimilation rate; relative water content
Subjects: in vitro culture; ex vitro acclimatization; irradiance; abscisic acid; growth; jasmonic acid; net photosynthetic rate; relative water content; salicylic acid; proline
Species: Ulmus minor

Received: January 7, 2013; Accepted: February 21, 2013; Published: December 1, 2013Show citation

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Dias, M.C., Pinto, G., Guerra, C., Jesus, C., Amaral, J., & Santos, C. (2013). Effect of irradiance during acclimatization on content of proline and phytohormones in micropropagated Ulmus minor. Biologia plantarum, 57(4), 769-772. doi: 10.1007/s10535-013-0341-1.
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