Biologia plantarum 58:64-70, 2014 | DOI: 10.1007/s10535-013-0351-z

Identification of new TRAP markers linked to chlorophyll content, leaf senescence, and cell membrane stability in water-stressed wheat

M. S. Saleh1, A. A. Al-Doss2, A. A. Elshafei2, K. A. Moustafa2, F. H. Al-Qurainy1, M. N. Barakat2,3,*
1 Botany and Microbiology Department, College of Pure Science, King Saud University, Riyadh, Riyadh, Saudi Arabia
2 Plant Production Department, College of Food and Agriculture Sciences, King Saud University, Riyadh, Riyadh, Saudi Arabia
3 Crop Science Department, Faculty of Agriculture, University of Alexandria, Alexandria, Egypt

In order to identify target region amplification polymorphism (TRAP) markers linked to three physiological traits in wheat (Triticum aestivum L.), the segregating F4 population from the cross between drought-sensitive (Yecora Rojo) and drought-tolerant (Pavon 76) genotypes was made. The parents and 150 F4 families were evaluated phenotypically for drought tolerance using two irrigation treatments [2.5 and 7.5 m3(H2O) m-2(soil)]. Using 40 different TRAP primer combinations tested for polymorphism in parental and F4 family genotypes, the results revealed that quantitative trait locus (QTL) for chlorophyll content was associated with TRAP 5, TRAP 14, and TRAP 20 and explained 18, 16, and 23 % phenotypic variation, respectively. The genetic distance between chlorophyll content QTL and TRAP 5, TRAP 14, and TRAP 20 were 12.3, 19.8, and 13.6 cM, respectively. QTL for flag leaf senescence was associated with TRAP 2, TRAP 3, TRAP 15, and TRAP 16 and explained 33, 27, 28, and 23 % phenotypic variations, respectively. The genetic distance between flag leaf senescence QTL and TRAP 2, TRAP 3, TRAP 15, and TRAP 16 were 9.4, 14.7, 18.1, and 17.3 cM, respectively. QTL for cell membrane stability was associated with TRAP 8, TRAP 9, and TRAP 37 and explained 27, 30, and 24 % phenotypic variation, respectively. The markers TRAP 8, TRAP 9, and TRAP 37 had genetic distances of 17.0, 10.0, and 9.0 cM, respectively. Therefore, these TRAP markers can be used in breeding for drought tolerance in wheat.

Keywords: drought tolerance; genetic distance; QTL; target region amplification polymorphism; Triticum aestivum
Subjects: TRAP markers; chlorophyll content; leaf senescence; cell membrane stability; water stress; drought tolerance; genetic distance; QTL; wheat
Species: Triticum aestivum

Received: January 27, 2013; Revised: April 6, 2013; Accepted: April 8, 2013; Published: March 1, 2014Show citation

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Saleh, M.S., Al-Doss, A.A., Elshafei, A.A., Moustafa, K.A., Al-Qurainy, F.H., & Barakat, M.N. (2014). Identification of new TRAP markers linked to chlorophyll content, leaf senescence, and cell membrane stability in water-stressed wheat. Biologia plantarum, 58(1), 64-70. doi: 10.1007/s10535-013-0351-z.
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