Biologia plantarum 58:697-708, 2014 | DOI: 10.1007/s10535-014-0447-0

Alleviation of osmotic stress in Brassica napus, B. campestris, and B. juncea by ascorbic acid application

M. M. Alam1, K. Nahar1,2, M. Hasanuzzaman3, M. Fujita1,*
1 Laboratory of Plant Stress Responses, Department of Applied Biological Science, Faculty of Agriculture, Kagawa University, Miki-cho, Kita-gun, Kagawa, Japan
2 Department of Agricultural Botany, Sher-e-Bangla Agricultural University, Sher-e-Bangla Nagar, Dhaka, Bangladesh
3 Sher-e-Bangla Agricultural University, Sher-e-Bangla Nagar, Dhaka, Bangladesh

The roles of ascorbic acid (AsA, 1 mM) under an osmotic stress [induced by 15 % (m/v) polyethylene glycol, PEG-6000] were investigated by examining morphological and physiological attributes in Brassica species. The osmotic stress reduced the fresh and dry masses, leaf relative water content (RWC), and chlorophyll (Chl) content, whereas increased the proline (Pro), malondialdehyde (MDA), and H2O2 content, and lipoxygenase (LOX) activity. The ascorbate content in B. napus, B. campestris, and B. juncea decreased, increased, and remained unaltered, respectively. The dehydroascorbate (DHA) content increased only in B. napus. The AsA/DHA ratio was reduced by the osmotic stress in all the species except B. juncea. The osmotic stress increased the glutathione (GSH) content only in B. juncea, but increased the glutathione disulfide (GSSG) content and decreased the GSH/GSSG ratio in all the species. The osmotic stress increased the activities of ascorbate peroxidase (APX) (except in B. napus), glutathione reductase (GR) (except in B. napus), glutathione S-transferase (GST) (except in B. juncea), and glutathione peroxidase (GPX), and decreased the activities of catalase (CAT) and monodehydroascorbate reductase (MDHAR) (only in B. campestris). The osmotic stress decreased the glyoxalase I (Gly I) and increased glyoxalase II (Gly II) activities. The application of AsA in combination with PEG improved the fresh mass, RWC, and Chl content, whereas decreased the Pro, MDA, and H2O2 content in comparison with PEG alone. The AsA addition improved AsA-GSH cycle components and improved the activities of all antioxidant and glyoxalase enzymes in most of the cases. So, exogenous AsA improved physiological adaptation and alleviated oxidative damage under the osmotic stress by improving the antioxidant and glyoxalase systems. According to measured parameters, B. juncea can be recognized as more drought tolerant than B. napus and B. campestris.

Keywords: abiotic stress tolerance; antioxidants; AsA-GSH cycle; methylglyoxal; oxidative stress; polyethylene glycol
Subjects: osmotic stress; ascorbic acid; glutathione; ascorbic-glutathione cycle; polyethylene glycol; relative water content; proline; chlorophyll; malondialdehyde; ascorbate peroxidase; glutathione reductase; catalase; glutathione peroxidase
Species: Brassica napus; Brassica campestris; Brassica juncea

Received: February 2, 2014; Revised: February 27, 2014; Accepted: March 3, 2014; Published: December 1, 2014Show citation

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Alam, M.M., Nahar, K., Hasanuzzaman, M., & Fujita, M. (2014). Alleviation of osmotic stress in Brassica napus, B. campestris, and B. juncea by ascorbic acid application. Biologia plantarum, 58(4), 697-708. doi: 10.1007/s10535-014-0447-0.
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