Biologia plantarum 59:183-186, 2015 | DOI: 10.1007/s10535-014-0458-x

Nitric oxide accumulation and glycinebetaine metabolism in two osmotically stressed maize cultivars supplied with different nitrogen forms

L. X. Zhang1, P. Zheng1, Z. Ruan1, L. Tian1, M. Ashraf2,*
1 College of Life Sciences, Northwest A&F University, Yangling, P.R. China
2 University College of Agriculture, University of Sargodha, Sargodha, Pakistan

Hydroponic experiments were conducted to investigate the effects of nitrogen forms on nitric oxide accumulation and glycinebetaine (GB) metabolism under osmotic stress (OS) simulated by the addition of polyethylene glycol (PEG-6000) to two maize (Zea mays L.) cultivars Zhengdan 958 (ZD958; drought-tolerant) and Jundan 20 (JD20; drought-sensitive). OS led to the accumulation of NO, GB, and choline, as well as to an increase of betaine aldehyde dehydrogenase (BADH) activity in both the cultivars. The responses of both the maize cultivars to OS were significantly higher under nitrate nutrition than under the mixed supply of ammonium and NO3 - or sole NH4 +. Cultivar ZD958 was superior to JD20 under the osmotic stress. Furthermore, compared with sole NH4 +, sole NO3 - and the mixed supply of NH4 + and NO3 - increased all the parameters in cv. ZD958 but only the GB and choline content in JD20. Therefore, it is concluded that ZD958 showed a higher accumulation of NO and enhanced GB metabolism under OS than JD20 regardless of N form.

Keywords: betaine aldehyde dehydrogenase; choline; drought stress; Zea mays
Subjects: nitric oxide; glycine betaine; choline; betaine aldehyde dehydrogenase; nitrogen forms; maize
Species: Zea mays

Received: April 11, 2014; Revised: June 22, 2014; Accepted: July 2, 2014; Published: January 1, 2015Show citation

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Zhang, L.X., Zheng, P., Ruan, Z., Tian, L., & Ashraf, M. (2015). Nitric oxide accumulation and glycinebetaine metabolism in two osmotically stressed maize cultivars supplied with different nitrogen forms. Biologia plantarum, 59(1), 183-186. doi: 10.1007/s10535-014-0458-x.
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