Biologia plantarum 45:299-302, 2002 | DOI: 10.1023/A:1015169311876

Plant Regeneration Through Somatic Embryogenesis in Leaf Derived Callus of Plumbago Rosea

G. Das1, G.R. Rout1
1 Plant Biotechnology Division, Plant Tissue Culture Laboratory, Regional Plant Resource Centre, Bhubaneswar, India

Regeneration of Plumbago rosea L., a rare medicinal plant, via somatic embryogenesis in callus cultures derived from leaf explants was described. Optimum callus formation was achieved on semi-solid Murashige and Skoog (MS) medium supplemented with 0.25 mg dm-3 kinetin and 2.0 mg dm-3 1-naphthaleneacetic acid (NAA). Somatic embryogenesis was achieved upon transferring the 4-week-old callus to a medium containing 1.0 mg dm-3 kinetic (Kn), 0.5 mg dm-3 gibberellic acid (GA3) and 0.1 mg dm-3 NAA. Embryo maturation and germination was achieved on the half-strength MS basal salts supplemented with 0.01 - 0.25 mg dm-3 Kn and 2 % (m/v) saccharose. An average of 50 - 60 plantlets were obtained from 150 mg of embryogenic callus within 4 week of subculture. Out of the 50 plantlets about 28 survived in the greenhouse.

Keywords: gibberellic acid; naphthaleneacetic acid; in vitro; medicinal plant
Subjects: auxins; gibberelins; in vitro culture, somatic embryogenesis; Plumbago rosea; somatic embryogenesis

Published: June 1, 2002Show citation

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Das, G., & Rout, G.R. (2002). Plant Regeneration Through Somatic Embryogenesis in Leaf Derived Callus of Plumbago Rosea. Biologia plantarum, 45(2), 299-302. doi: 10.1023/A:1015169311876.
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    References

    1. Čulafić, L., Budirmir, S., Vujicić, R., Nešković, M.: Induction of somatic embryogenesis and embryo development in Rumex acetosella L.-Plant Cell Tissue Organ Cult. 11: 133-139, 1987.
    2. Chang, W.C., Hsing, Y.: Plant regeneration through somatic embryogenesis in root derived callus of ginseng (Panax ginseng A. Meyer).-Theor. appl. Genet. 57: 133-135, 1980. Go to original source...
    3. Chetia, S., Handique, P.J.: High frequency in vitro shoot multiplication of Plumbago indica, a rare medicinal plant.-Curr. Sci. 78: 1187-1188, 2000.
    4. Davey, M.R., Power, J.B.: Aspects of protoplast culture and plant regeneration.-Plant Cell Tissue Organ Cult. 12: 115-125, 1988. Go to original source...
    5. Harter, H.L.: Critical values for Duncan's multiple range test.-Biometrics 16: 671-685, 1960. Go to original source...
    6. Ignacimuthu, S., Arockiasamy, S., Antonysamy, M., Ravichandran, P.: Plant regeneration through somatic embryogenesis from mature leaf explants of Eryngium foetidum, a condiment.-Plant Cell Tissue Organ Cult. 56: 131-137, 1999. Go to original source...
    7. Kunitake, H., Mii, M.: Somatic embryogenesis and plant regeneration from protoplasts of asparagus (Asparagus officinalis L.).-Plant Cell Rep. 8: 706-710, 1990. Go to original source...
    8. Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures.-Physiol. Plant. 15: 473-497, 1962. Go to original source...
    9. Rout, G.R, Debata, B.K., Das, P.: Somatic embryogenesis in callus cultures of Rosa hybrida cv. Landora.-Plant Cell Tissue Organ Cult. 27: 65-69, 1991. Go to original source...
    10. Rout, G.R., Das, P.: Somatic embryogenesis in Simarouba glauca L.-Plant Cell Tissue Organ Cult. 37: 79-81, 1994. Go to original source...
    11. Rout, G.R., Saxena, C., Samantaray, S., Das, P.: Rapid clonal propagation of Plumbago zeylanica Linn.-Plant Growth Regul. 28: 1-4, 1999a. Go to original source...
    12. Rout, G.R, Saxena, C., Samantaray, S., Das, P.: Rapid plant regeneration from callus cultures of Plumbago zeylanica. Plant Cell Tissue Organ Cult. 56: 47-51, 1999b. Go to original source...
    13. Sathesh Kumar, K., Bhavanandan, K.V.: Micropropagation of Plumbago rosea Linn.-Plant Cell Tissue Organ Cult. 15: 275-278, 1988. Go to original source...
    14. Saxena, C., Samantaray, S., Rout, G.R., Das, P.: Effect of auxins on in vitro rooting of Plumbago zeylanica: peroxidase activity as a marker for root induction.-Biol. Plant. 43: 121-124, 2000. Go to original source...
    15. Xiao, X.G., Branchard, M.: Embryogenesis and plant regeneration of spinach (Spinacia oleracea L.) from hypocotyl segments.-Plant Cell Rep. 13: 69-71, 1983. Go to original source...

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