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Biologia plantarum 50:146-148, 2006 | DOI: 10.1007/s10535-005-0091-9

Radiation as a tool to remove selective marker genes from transgenic soybean plants

M. L. Tinoco1,3, G. R. Vianna1, S. Abud2, P. I. M. Souza2, E. L. Rech1, F. J. L. Aragao1,*
1 Embrapa Recursos Geneticos e Biotecnologia, Brasilia, DF., Brazil
2 Embrapa Cerrados, Rod. Brasilia-Fortaleza (BR 020), Planaltina, DF., Brazil
3 Departamento de Biologia Celular, Universidade de Brasilia, Brasilia, DF., Brazil

The present study evaluated the use of γ-radiation to physically remove selective marker genes previously introduced into the soybean genome. Homozygous seeds from a transgenic soybean line carrying the gus and ahas transgenes were irradiated with γ-rays. Six plants presenting a deleted gus gene were analyzed by Southern blot to confirm removal of both ahas and gus genes. Line 1A presented an absence of the gus gene cassette and presence of the ahas gene cassette.

Keywords: γ-radiation; genetically modified plant; Glycine max; mutation
Subjects: photosystem 2; radiation gamma; transgenic plants (cf. also in vitro…)

Received: April 25, 2004; Accepted: October 11, 2004; Published: March 1, 2006Show citation

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