Biologia plantarum 46:289-292, 2003 | DOI: 10.1023/B:BIOP.0000022268.34748.d8

Efficient Haploid Induction in Microspore Suspension Culture of Aesculus hippocastanum and Karyotype Analysis

D. Ćalić1, S. Zdravković-Korać1, S. Jevremović1, M. Guć-Šćekić2, Lj. Radojević1
1 Department of Plant Physiology, Institute for Biological Research, "Siniša Stanković", Belgrade
2 Department of Genetics, Institute for Mother and Child Health Care, Belgrade

Suspension culture was more efficient method for haploid production than anther culture. All analysed androgenic regenerants originating from embryogenic microspores in suspension culture of Aesculus hippocastanum L. had a haploid number of chromosomes (n=20), while 50 % of those derived from anther culture were diploids.

Keywords: androgenesis; anther culture; horse chestnut
Subjects: Aesculus hippocastanum; androgenesis; haploid, efficient induction in microspore culture; horse chestnut, androgenic regenerants; in vitro culture, microspore suspension culture; microspore, suspension culture

Published: September 1, 2003Show citation

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Ćalić, D., Zdravković-Korać, S., Jevremović, S., Guć-Šćekić, M., & Radojević, L. (2003). Efficient Haploid Induction in Microspore Suspension Culture of Aesculus hippocastanum and Karyotype Analysis. Biologia plantarum, 46(2), 289-292. doi: 10.1023/B:BIOP.0000022268.34748.d8.
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