Biologia plantarum 57:701-710, 2013 | DOI: 10.1007/s10535-013-0330-4

Characterization of a wheat C2 domain protein encoding gene regulated by stripe rust and abiotic stresses

G. Zhang1, Y. F. Sun1, Y. M. Li1, Y. L. Dong1, X. L. Huang1, Y. T. Yu1, J. M. Wang1, X. M. Wang1, X. J. Wang1, Z. S. Kang1,*
1 State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest A&F University, Yangling, Shaanxi, P.R. China

Plant C2 domain proteins play important roles in diverse cellular processes including growth, development, and membrane targeting, as well as in abiotic and biotic stress adaptations by sensing intracellular Ca2+ signals. In this study, we isolated a novel C2 domain protein gene, TaERG3, from wheat infected by Puccinia striiformis f. sp. tritici. TaERG3 was predicted to encode a 144 amino acid protein with molecular mass of 15.68 kD and isoelectric point of 3.93. Analysis of the deduced amino acid sequence of TaERG3 using InterProScan revealed the presence of an N-terminal calciumdependent phospholipid-binding module (C2 domain, 5 to 103). Transient expression analysis showed that the TaERG3 protein was predominately and uniformly localized in the plasmalemma and nucleus of onion epidermal cells. Quantitative real-time PCR analyses indicated that TaERG3 transcript was differentially induced in both incompatible and compatible interactions, as well as by applied abscisic acid (ABA) and CaCl2. However, the significant transcript changes induced by methyl jasmonate, ethylene, and salicylic acid treatments were not as dramatic as those induced by ABA. TaERG3 was also up-regulated by environmental stimuli including low temperature and high salinity. These results imply that TaERG3 might be involved in wheat defence responses against stripe rust and abiotic stresses in an ABA-dependent signalling pathway.

Keywords: abscisic acid; ethylene; expression pattern; low temperature; methyl jasmonate; Puccinia striiformis; salicylic acid; salinity; Triticum aestivum
Subjects: C2 domain proteins; gene expression; abscisic acid; ethylene; temperature - low; methyl jasmonate; salicylic acid; amino acid sequences; compatible reaction; incompatible reaction; calcium; salinity; phylogenetic tree; green fluorescent protein; wheat; stripe rust; onion
Species: Triticum aestivum; Puccinia striiformis; Allium cepa

Received: September 25, 2012; Accepted: January 21, 2013; Published: December 1, 2013Show citation

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Zhang, G., Sun, Y.F., Li, Y.M., Dong, Y.L., Huang, X.L., Yu, Y.T., ... Kang, Z.S. (2013). Characterization of a wheat C2 domain protein encoding gene regulated by stripe rust and abiotic stresses. Biologia plantarum, 57(4), 701-710. doi: 10.1007/s10535-013-0330-4.
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