Biologia plantarum 61:483-493, 2017 | DOI: 10.1007/s10535-016-0682-7

Identification of alternatively spliced MsRan transcripts involved in low temperature response in Musa spp.

Y. L. Zhang1, Z. Z. Fang2, Z. X. Lai1,*
1 Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, Fuzhou, Fujian, P.R. China
2 Fruit Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian, P.R. China

Ran is involved in response to external stimuli. In this study, six MsRan gene cDNA sequences were isolated from wild banana (Musa spp. AB group) from Sanming City, China. Sequence analysis reveals that MsRan3A, MsRan3A-1a, and MsRan3C contained Ran protein domains including a GTP hydrolysis domain, a RanGAP-binding domain, and an acidic tail, whereas two G boxes (G4 and G5) were absent in MsRan3A-6a. The physicochemical property of MsRan3A, MsRan3A-1a, MsRan3A-6a, and MsRan3C appeared to differ significantly. Real time quantitative PCR (qPCR) analysis indicates that MsRan3A-1, MsRan3A-5, MsRan3A-6, MsRan3A-6a, and MsRan3C-1 were expressed in roots, leaves, peduncles, bracts, flowers, peels, and pulp of the wild banana. MsRan3A-1a was expressed at extremely low levels in these tissues and was undetectable by qPCR. The MsRan genes were found to be involved in responses to a low temperature stress but with different response patterns. Furthermore, salicylic acid significantly enhanced MsRan gene expressions suggesting the involvement of these genes in salicylic acid signal transduction.

Keywords: gene expression; salicylic acid; wild banana
Subjects: MsRan transcripts; low temperature; gene expression; salicylic acid; splicing patterns; phylogenetic tree; wild banana
Species: Musa spp.

Received: September 20, 2015; Revised: June 28, 2016; Accepted: July 12, 2016; Published: September 1, 2017Show citation

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Zhang, Y.L., Fang, Z.Z., & Lai, Z.X. (2017). Identification of alternatively spliced MsRan transcripts involved in low temperature response in Musa spp. Biologia plantarum, 61(3), 483-493. doi: 10.1007/s10535-016-0682-7.
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