Biologia plantarum 48:101-104, 2004 | DOI: 10.1023/B:BIOP.0000024282.01087.a3

Shoot Regeneration in Four Begonia Genotypes

F.J. Espino1, R. Linacero1, J. Rueda1, A.M. Vázquez1,*
1 Departamento de Genêtica, Facultad de Biología, Universidad Complutense de Madrid, Madrid, Spain

In vitro regeneration of four Begonia genotypes, B. semperflorens, B. rex, B.×elatior, and hybrid of Begonia with unknown parents 'Tiger' was carried out starting from leaf and petiole segments as explants. Five Murashige and Skoog's derived media were tested, three of them supplemented with α-naphthaleneacetic acid (NAA) and 6-benzyladenine (BA), and the other two with NAA and kinetin (KIN) in different concentrations. Shoot regeneration was preferentially induced on the BA containing media, quantitative differences being observed among explants and genotypes.

Keywords: explant type; genotype effect; growth regulators; in vitro culture; organogenic response
Subjects: auxins; Begonia × elatior (= B. hiemalis); Begonia rex; Begonia semperflorens; Begonia 'Tiger'; cultivar and genotype differences, shoot regeneration; cytokinins; genotypes, regeneration; in vitro culture, nutrient media modification; in vitro culture, regeneration, organogenesis; nutrient medium, Murashige and Skoog

Published: March 1, 2004Show citation

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Espino, F.J., Linacero, R., Rueda, J., & Vázquez, A.M. (2004). Shoot Regeneration in Four Begonia Genotypes. Biologia plantarum, 48(1), 101-104. doi: 10.1023/B:BIOP.0000024282.01087.a3.
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    References

    1. Babber, S., Mittal, K., Ahlawat, R., Varghese, T.M.: Micropropagation of Cardiospermum halicacabum.-Biol. Plant. 44: 603-606, 2001. Go to original source...
    2. Burrit, D.J., Leung, D.W.M.: Organogenesis in cultured petiole explants of Begonia x erythrophylla: the timing and specificity of the inductive stimuli.-J. exp. Bot. 47: 557-567, 1996. Go to original source...
    3. Cassells, A.C., Morrish, F.M.: Variation in adventitious regenerants of Begonia rex Putz. 'Lucille Closon' as a consequence of cell ontogeny, callus ageing and frequency of callus sub-culture.-Scientia Hort. 32: 135-143, 1987. Go to original source...
    4. Chlyah, A., Tran Thanh Van, M.: Differential reactivity in epidermal cells of Begonia rex excised and grown in vitro.-Physiol. Plant. 35: 16-20, 1975. Go to original source...
    5. Khoder, M., Villemur, P., Jonard, R.: Vegetative propagation of the Elatior begonia cv. Rieger from different organs grown in vitro.-Compt. rend. Acad. Sci. Paris Sêr. III 293: 403-408, 1981.
    6. Kishimoto, S., Aida, R., Shibata, M.: Agrobacterium tumefaciens-mediated transformation of Elatior Begonia (Begonia x hiemalis Fotsch).-Plant Sci. 162: 697-703, 2002. Go to original source...
    7. Kiyokawa, T., Kikuchi, Y., Kamada, H., Harada, H.: Genetic transformation of Begonia tuberhybrida by Ri rol genes.-Plant Cell Rep. 15: 606-609, 1996. Go to original source...
    8. Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures.-Physiol. Plant. 15: 473-497, 1962. Go to original source...
    9. Saritha, K.V., Prakash, E., Ramamurthy, N., Naidu, C.V.: Micropropagation of Spilanthes acmella Murr.-Biol. Plant. 45: 581-584, 2002. Go to original source...
    10. Simmonds, J.: Induction, growth and direct rooting of adventitious shoots of Begonia x hiemalis.-Plant Cell Tissue Organ Cult. 3: 283-289, 1984. Go to original source...
    11. Takayama, S.: Begonia.-In: Amirato, P.V., Evans, D.A., Sharp, W.R., Bajaj, Y.P.S. (ed.): Handbook of Plant Cell Culture. Vol. 5. Pp. 253-282. McGraw-Hill Publ. Co., New York 1990.

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