Biologia plantarum 56:566-570, 2012 | DOI: 10.1007/s10535-012-0118-y

Effects of 5-aminolevulinic acid on the H2O2-content and antioxidative enzyme gene expression in NaCl-treated cucumber seedlings

A. Zhen1, Z. L. Bie1,*, Y. Huang1, Z. X. Liu1, M. L. Fan1
1 College of Horticulture and Forestry, Huazhong Agricultural University and Key Laboratory of Horticultural Plant Biology, Ministry of Education, Wuhan, P.R. China

The potential of 5-aminolevulenic acid (ALA) to enhance the salt tolerance of cucumber (Cucumis sativus L.) seedlings was investigated. ALA was applied at various concentrations (0, 1, 10, 25, 50, and 100 mg dm-3) as foliar spray or root watering. Then the seedlings were exposed to 0 or 75 mM NaCl for 5 d. NaCl stress reduced the root and leaf dry masses, leaf area, and the leaf net CO2 assimilation rate. These reductions were counteracted by exogenous ALA, and the most efficient was 50 mg dm-3 concentration via foliar spray. ALA decreased the H2O2 contents and increased the activities of ascorbate peroxidase (APX) and glutathione reductase (GR) in NaCl-treated cucumber roots and leaves and the activity of catalase (CAT) in leaves. The ALA application also up-regulated the expressions of CAT and cAPX genes in roots and leaves and the expression of GR gene in roots of the NaCl treated cucumber plants.

Keywords: ascorbate peroxidase; catalase; Cucumis sativus; gene expression; glutathione reductase; salinity
Subjects: ascorbate peroxidase; catalase; gene expression; glutathione reductase; aminolevulinic acid; hydrogen peroxide; NaCl; PCR; net photosynthetic rate; superoxide dismutase; ascorbate; glutathione
Species: Cucumis sativus

Received: February 23, 2011; Accepted: May 31, 2011; Published: September 1, 2012Show citation

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Zhen, A., Bie, Z.L., Huang, Y., Liu, Z.X., & Fan, M.L. (2012). Effects of 5-aminolevulinic acid on the H2O2-content and antioxidative enzyme gene expression in NaCl-treated cucumber seedlings. Biologia plantarum, 56(3), 566-570. doi: 10.1007/s10535-012-0118-y.
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    References

    1. Aghaleh, M., Niknam, V., Ebrahimzadeh, H., Razavi, K.: Salt stress effects on growth, pigments, proteins and lipid peroxidation in Salicornia persica and S. europaea. - Biol. Plant. 53: 243-248, 2009. Go to original source...
    2. Asada, K.: Production and scavenging of reactive oxygen species in chloroplasts and their functions. - J. Plant Physiol. 141: 391-396, 2006. Go to original source...
    3. Ashraf, M.: Biotechnological approach of improving plant salt tolerance using antioxidants as markers. - Biotechnol. Adv. 27: 84-93, 2009. Go to original source...
    4. Attia, H., Arnaud, N., Karray, N., Lachaâl, M.: Long-term effects of mild salt stress on growth, ion accumulation and superoxide dismutase expression on Arabidopsis rosette leaves. - Physiol. Plant. 132: 293-305, 2008. Go to original source...
    5. Brennan, T., Frenkel, C.: Involvement of hydrogen peroxide in the regulation of senescence in pear. - Plant Physiol. 59: 411-416, 1977. Go to original source...
    6. Castillo, F.J., Greppin, H.: Extracellular ascorbic acid and enzyme activities related to ascorbic acid metabolism in Sedum album L. leaves after ozone exposure. - Environ. exp. Bot. 28: 232-233, 1988. Go to original source...
    7. Chen, J.X., Wang, X.F.: [Experimental Instruction of Plant Physiology.] Pp. 12-127. South China University of Thechnology Press, Guangzhou 2002 [in Chinese].
    8. Dhindsa, R.S., Plumb-Dhindsa, P., Thorpe, T.A.: Leaf senescence: correlated with increased levels of membrane permeability and lipid peroxidation, and decreased levels of superoxide dismutase and catalase. - J. exp. Bot. 32: 93-101, 1981. Go to original source...
    9. Foyer, C.H., Halliwell, B.: Presence of glutathione and glutathione reductase in chloroplast: a proposed role on ascorbic acid metabolism. - Planta 133: 21-25, 1976. Go to original source...
    10. He, Y., Zhu, Z.J., Exogenous salicylic acid alleviates NaCl toxicity and increases antioxidative enzyme activity in Lycopersicon esculentum. - Biol. Plant. 52: 792-795, 2008. Go to original source...
    11. Hernandez, M., Fernandez-Garcia, N., Diaz-Vivancos, P., Olmos, E.: A different role for hydrogen peroxide and the antioxidative system under short and long salt stress in Brassica oleracea roots. - J. exp. Bot. 61: 521-535, 2010. Go to original source...
    12. Hoagland, D.R., Arnon, D.S.: The water culture method for growing plants without soil. - Calif. Agr. Exp. Sta. Circular 347: 1-32, 1950.
    13. Hossain, M.A., Nakano, Y., Asada, K.: Monodeydroascorbate reductase in spinach chloroplast and its participation in regeneration of ascorbate for scavenging hydrogen peroxide. - Plant Cell Physiol. 25: 385-395, 1984.
    14. Huang, Y., Tang, R., Cao, Q.L., Bie, Z.L.: Improving the fruit yield and quality of cucumber by grafting onto the salt tolerant rootstock under NaCl stress. - Sci. Hort. 122: 26-31, 2009. Go to original source...
    15. Kang, G.., Wang, C., Sun, G., Wang, Z.: Salicylic acid changes activities of H2O2-metabolizing enzymes and increases the chilling tolerance of banana seedlings. - Environ. exp. Bot. 50: 9-15, 2003. Go to original source...
    16. Kholová, J., Sairam, R.K., Meena, R.C., Srivastava, G.C.: Response of maize genotypes to salinity stress in relation to osmolytes and metal-ion contents, oxidative stress and antioxidant enzymes activity. - Biol. Plant. 53: 249-256, 2009. Go to original source...
    17. Kim, S.Y., Lim, J.-H., Park, M.R., Kim, Y.J., Park, T.H., Sco, Y.W., Choi, K.G., Yun, S.J.: Enhanced antioxidant enzymes are associated with reduced hydrogen peroxide in barley roots under saline stress. - J. Biochem. mol. Biol. 38: 218-224, 2005. Go to original source...
    18. Korkmaz, A., Korkmaz, Y., Demirkiran, A.R.: Enhancing chilling stress tolerance of pepper seedlings by exogenous application of 5-aminolevulinic acid. - Environ. exp. Bot. 67: 495-501, 2010. Go to original source...
    19. Liu, Y.J.: Experiment Technology of Plant Biochemistry and Physiology. Pp. 146-147. Agriculture of China Press, Beijing 2000.
    20. Mallik, S., Nayak, M., Sahu, A.K., Shaw B.P.: Response of antioxidant enzymes to high NaCl concentration in different salt-tolerant plants. - Biol. Plant. 55: 191-195, 2011. Go to original source...
    21. Mandhania, S., Madan, S., Sawhney, V.: Antioxidant defense mechanism under salt stress in wheat seedlings. - Biol. Plant. 50: 227-231, 2006. Go to original source...
    22. Meloni, D.A., Oliva, M.A., Martinez, C.A., Cambraia, J.: Photosynthesis and activity of superoxide dismutase, peroxidase and glutathione reductase in cotton under salt stress. - Environ. exp. Bot. 49: 69-76, 2003. Go to original source...
    23. Memon, S.A., Hou, X., Wang, L., Li, Y.: Promotive effect of 5-aminolevulinic acid on chlorophyll, antioxidative enzymes and photosynthesis of pakchoi (Brassica campestris ssp. chinensis var. communis Tsen et Lee). - Acta Physiol. Plant. 31: 51-57, 2009. Go to original source...
    24. Mulholland, B.J., Taylor, I.B., Jackson, A.C., Thompson, A.J.: Can ABA mediate responses of salinity stressed tomato. - Environ. exp. Bot. 50: 17-28, 2003. Go to original source...
    25. Naeem, M.S., Jin, Z.L., Wan, G.L., Liu, D., Liu, H.B., Yoneyama, K., Zhou, W.J.: 5-Aminolevulinic acid improves photosynthetic gas exchange capacity and ion uptake under salinity stress in oilseed rape (Brassica napus L.). - Plant Soil 332: 405-415, 2010. Go to original source...
    26. Nakano, Y., Asada, K.: Hydrogen peroxide scanvenged by ascorbate-specific peroxidase in spinach chloroplast. - Plant Cell Physiol. 22: 867-880, 1981.
    27. Nishihara, E., Kondo, K., Parvez, M.M., Takahashi, K., Watanabe, K., Tanaka, K.: Role of 5-aminolevulinic acid (ALA) on active oxygen-scavenging system in NaCl-treated spinach (Spinacia oleracea). - J. Plant Physiol. 160: 1085-1091, 2003. Go to original source...
    28. Shaw, B.P., Sahu, S.K., Mishra, R.K.: Heavy metal induced oxidative damage in terrestrial plants. - In: Prasad, M.N.V. (ed.): Heavy Metals Stress in Plants: from Biomolecules to Ecosystems. Pp. 84-145. Springer-Verlag, Heidelberg 2004. Go to original source...
    29. Stępień, P., KŁobus, G.: Water relations and photosynthesis in Cucumis sativus L. leaves under salt stress. - Biol. Plant. 50: 610-616, 2006.
    30. Wang, J.J., Jiang, W.B., Liu, H., Liu, W.Q., Kang, L., Hou, X.L.: Promotion by 5-aminolevulinic acid of germination of pakchoi (Brassica campestris ssp. chinensis var. communis Tsen et Lee) seeds under salt stress. - J. Integ. Plant Biol. 47: 1084-1091, 2005. Go to original source...
    31. Wang, L.J., Jiang, W.B., Zhang, Z., Yao, Q.H., Matsui, H., Ohara, H.: 5-Aminolevinilic acid and its potential application in agriculture. - Plant Physiol. Commun. 39: 185-192, 2003.
    32. Warren, G.J.: Responses to low temperature and adaptation to freezing. - In: Malcolm, J.H., Peter, B. (ed.): Molecular Analysis of Plant Adaptation to the Environment. Pp. 209-247. Kluwer Academic Publishers, Dordrecht 2001. Go to original source...
    33. Watanabe, K., Nishihara, E., Watanabe, S., Tanaka, T., Takahashi, K., Takeuchi, Y.: Enhancement of growth and fruit maturity in 2-year-old grapevines cv. Delaware by 5-aminolevulinic acid. - Plant Growth Regul. 49: 35-42, 2006. Go to original source...
    34. Zhang, Z.J., Li, H.Z., Zhou, W.J., Takeuchi, Y., Yoneyama, K.: Effect of 5-aminolevulinic acid on development and salt tolerance of potato (Solanum tuberosum L.) microtubers in vitro. - Plant Growth Regul. 49: 27-34, 2006.
    35. Zhu, J., Bie, Z.L., Li, Y.N.: Physiological and growth responses of two different salt-sensitive cucumber cultivars to NaCl stress. - Soil Sci. Plant Nutr. 54: 400-407, 2008. Go to original source...
    36. Zuccarini, P.: Effects of silicon on photosynthesis, water relations and nutrient uptake of Phaseolus vulgaris under NaCl stress. - Biol. Plant. 52: 157-160, 2008. Go to original source...

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