Biologia plantarum 62:277-286, 2018 | DOI: 10.1007/s10535-018-0779-2

Identification of the genes involved in heterotrimeric G-protein signaling in mulberry and their regulation by abiotic stresses and signal molecules

C. Y. Liu1, Y. Z. Xu1, W. Fan1, D. P. Long1, B. N. Cao1, Z. H. Xiang1, A. C. Zhao1,*
1 State Key Laboratory of Silkworm Genome Biology, Key Laboratory of Sericultural Biology and Genetic Breeding, Ministry of Agriculture, Southwest University, Chongqing, P.R. China

Heterotrimeric guanine-nucleotide-binding proteins (G-proteins) play important roles in signal transduction and regulate responses to various stresses. Although G-protein signaling pathways have been extensively identified and characterized in model plants, there is little knowledge in non-model and especially in woody plants. Mulberry is an economically and ecologically important perennial tree, which is adaptable to many environmental stresses. In this study, we identified and cloned six G-protein genes including one Gα, one Gβ, two Gγ, one RGS (regulator of G-protein signaling protein) and one RACK1 (receptor for activated C kinase 1) involved in G-protein signaling. Sequence and phylogenetic analysis revealed that Morus G-proteins are evolutionarily conserved compared with those of other plants. A real-time quantitative reverse transcription polymerase chain reaction analysis showed that Morus G-protein signaling genes were ubiquitously but differentially expressed in various tissues. The expression of all of these genes was affected by abiotic stresses and signal molecules, which indicated that Morus G-protein signaling may be involved in environmental stress and defense responses.

Keywords: biotic stress; expression profile; Morus atropurpurea; Morus notabilis
Subjects: G-protein; gene expression; amino acid sequences; phylogenetic analysis; anther culture; cold; drought; salinity
Species: Morus notabilis; Morus atropurpurea

Received: April 15, 2017; Revised: August 26, 2017; Accepted: August 28, 2017; Published: June 1, 2018Show citation

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Liu, C.Y., Xu, Y.Z., Fan, W., Long, D.P., Cao, B.N., Xiang, Z.H., & Zhao, A.C. (2018). Identification of the genes involved in heterotrimeric G-protein signaling in mulberry and their regulation by abiotic stresses and signal molecules. Biologia plantarum, 62(2), 277-286. doi: 10.1007/s10535-018-0779-2.
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