biologia plantarum

Biologia plantarum 53:243-248, 2009 | DOI: 10.1007/s10535-009-0046-7

Salt stress effects on growth, pigments, proteins and lipid peroxidation in Salicornia persica and S. europaea

M. Aghaleh¹, V. Niknam^1,*, H. Ebrahimzadeh¹, K. Razavi²

¹ School of Biology, University College of Science, University of Tehran, Tehran, I.R. Iran

² Department of Plant Biotechnology, National Institute for Genetic Engineering and Biotechnology, Tehran, I.R. Iran

The effects of NaCl stress on growth, water status, contents of protein, proline, malondialdehyde (MDA), various sugars and photosynthetic pigments were investigated in seedlings of Salicornia persica and S. europaea grown in vitro. Seeds were germinated under NaCl (0, 100, 200, 300, 400, 500 and 600 mM) on Murashige and Skoog medium for 45 d. The shoot growth of both species increased under low NaCl concentration (100 mM) and then decreased with increasing NaCl concentrations. In contrast to S. persica, root length in S. europaea reduced steadily with an increase in salinity. Proline content in S. persica was higher than in S. europaea at most NaCl concentrations. Proline, reducing saccharide, oligosaccharide and soluble saccharide contents increased under salinity in both species. In contrast, contents of proteins and polysaccharides reduced in both species under salt stress. MDA content remained close to control at moderate NaCl concentrations (100 and 200 mM) and increased at higher salinities. MDA content in S. europaea was significantly higher than S. persica at higher salinities. Salt treatments decreased K⁺ and P contents in seedlings of both species. Significant reduction in contents of chlorophylls and carotenoids due to NaCl stress was also observed in seedlings of both species. Some differences appeared between S. persica and S. europaea concerning proteins profile. On the basis of the data obtained, S. persica is more salt-tolerant than S. europaea.

Keywords: halophyte; in vitro culture; free proline; MDA; photosynthetic pigment; RWC; salinity; saccharides

Subjects: carotenoids; chlorophyll a,b; growth and growth analysis; in vitro culture, salinity; malondialdehyde; osmotic potential; phosphorus; potassium; proline; proteins; relative water content (RWC); Salicornia europea; Salicornia persica; salinity; sodium; sugars

Received: March 29, 2007; Accepted: January 20, 2008; Published: June 1, 2009Show citation