Biologia plantarum 46:295-296, 2003 | DOI: 10.1023/A:1022823315768

Relationship between Soil Nitrate Content and Activities of NADH: and NAD(P)H:Nitrate Reductases in Indian Mustard

A. Ahmad1, I. Khan2, Y.P. Abrol1, M.Z. Abdin2
1 Division of Plant Physiology, Indian Agricultural Research Institute, New Delhi-, India
2 Centre for Biotechnology, Hamdard University, New Delhi-, India

The pattern of NADH- and NAD(P)H-specific nitrate reductase (NRs) activities in Indian mustard (Brassica juncea L. Czern. and Coss.) was monitored throughout growth stages. NAD(P)H:NR (EC 1.6.6.2) activity was maximum at early stages of growth (30 days after sowing, DAS), then declined gradually reaching to almost zero at 90 DAS. Contrary to this, NADH:NR (EC 1.6.6.1) activity was low at 30 DAS, then gradually increased till 90 DAS and thereafter, it became constant. The decrease in NAD(P)H:NR activity and increase in the NADH:NR activity were associated with the seasonal decrease in nitrate content in the soil.

Keywords: Brassica juncea L.; growth stages
Subjects: Brassica juncea; Indian mustard, nitrate reductases; NADH:nitrate reductase, NAD(P)H:nitrate reductase; nitrate content in soil, nitrate reductases; nitrate reductase

Prepublished online: March 1, 2003; Published: September 1, 2003Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
Ahmad, A., Khan, I., Abrol, Y.P., & Abdin, M.Z. (2003). Relationship between Soil Nitrate Content and Activities of NADH: and NAD(P)H:Nitrate Reductases in Indian Mustard. Biologia plantarum46(2), 295-296. doi: 10.1023/A:1022823315768.
Download citation

References

  1. Abdin, M.Z., Kaim, M.S., Bansal, K.C., Abrol, Y.P.: Nitrate assimilation in leaf blades and N-harvest in uniculm 'Gigas' wheat.-J. Agron. Crop Sci. 173: 225-229, 1994. Go to original source...
  2. Ahmad, A., Abdin, M.Z.: NADH:nitrate reductase and NAD(P)H:nitrate reductase activities in mustard seedlings.-Plant Sci. 143: 1-7, 1999. Go to original source...
  3. Ahmad, A. Abrol, Y.P., Abdin, M.Z.: Effect of split application of sulphur and nitrogen on growth and yield attributes of Brassica genotypes differing in their time of flowering.-Can. J. Plant Sci. 79: 175-180, 1999.
  4. Bradford, M.M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.-Anal. Biochem. 72: 248-254, 1976. Go to original source...
  5. Evans, H.J., Nason, A.: Pyridine nucleotide nitrate reductase from extract of higher plants.-Plant Physiol. 28: 233-253, 1953. Go to original source...
  6. Fishman, M.J., Skougstad, M.W., Scarbro, G.F., Jr.: Determination of nitrate content of water.-J. amer. Water Works Assoc. 56: 633, 1964. Go to original source...
  7. Grover, H.L., Nair, T.V.R., Abrol, Y.P.: Nitrogen metabolism of the upper three leaf blades of wheat at different soil nitrogen levels. I. Nitrate reductase activity and content of various nitrogenous constituents.-Physiol. Plant. 42: 287-292, 1978. Go to original source...