Biologia plantarum 46:569-574, 2003 | DOI: 10.1023/B:BIOP.0000041063.38176.4a

Selecting Bacterial Strains for Use in the Biocontrol of Diseases Caused by Phytophthora capsici and Alternaria alternata in Sweet Pepper Plants

A. Sid1, M. Ezziyyani1, C. Egea-Gilabert2, M.E. Candela1
1 Department of Plant Physiology, University of Murcia, Espinardo, Murcia, Spain
2 Department of Agrarian Production, E.T.S.I.A., Polytechnic University of Cartagena, Cartagena, Spain

More than 500 isolates of bacteria were obtained from the aerial part and rhizosphere of sweet pepper (Capsicum annuum L.) plants harvested from different places in the Region of Murcia (Spain). The isolates were purified and assayed in vitro against Phytophthora capsici and Alternaria alternata. Sixty isolates (12 %) produced an inhibition zone against at least one of the pathogens, while ten had a strongly inhibitory effect on both pathogens assayed. Microscopic observation of interactions zone showed cell vacuolisation, hyphae lysis and spilling of cytoplasm content of the pathogens in the culture media. These ten isolates were then chosen for biocontrol of Phytophthora root rot and Alternaria leaf spots of pepper plants in vivo. Four of them denominated HS93, LS234, LS523 and LS674 reduced P. capsici root rot by 80, 51, 49 and 54 %, respectively, and A. alternata leaf spots by 54, 74, 62 and 53 %. HS93 belongs to the genus Bacillus and probably the species subtilis, while LS234, LS523 and LS674 belong to the genus Bacillus and probably the species licheniformis. Dry mass of plants treated with these bacteria was significantly higher than that of non-treated and inoculated plants.

Keywords: antagonism; plant growth-promoting rhizobacteria; Capsicum annuum
Subjects: Alternaria alternata; Bacillus licheniformis; Bacillus subtilis; bacterial strains for biocontrol of diseases; Capsicum annuum; pepper, biocontrol of diseases; Phytophthora capsici; resistance/tolerance to pathogens; rhizobacteria, plant growth-promoting; root rot of pepper; sweet pepper, biocontrol of diseases; sweet potato, biocontrol of diseases

Published: December 1, 2003Show citation

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Sid, A., Ezziyyani, M., Egea-Gilabert, C., & Candela, M.E. (2003). Selecting Bacterial Strains for Use in the Biocontrol of Diseases Caused by Phytophthora capsici and Alternaria alternata in Sweet Pepper Plants. Biologia plantarum, 46(4), 569-574. doi: 10.1023/B:BIOP.0000041063.38176.4a.
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