biologia plantarum

Biologia plantarum 52:713-717, 2008 | DOI: 10.1007/s10535-008-0137-x

Efficient regeneration of Eucommia ulmoides from hypocotyl explant

R. Chen^1,*, S. Namimatsu¹, Y. Nakadozono¹, T. Bamba¹, Y. Nakazawa¹, K. Gyokusen²

¹ Technical Research Institute, Hitachi Zosen Corporation, Osaka, Japan

² Department of Forest and Forest Products Sciences, Faculty of Agriculture, Kyushu University, Fukuoka, Japan

A rapid and efficient method for the regeneration of Eucommia ulmoides Oliver has been developed. The ability of hypocotyl segments to produce adventitious buds varied depending upon their position, seedling age and culture medium. The most adventitious buds were induced from the hypocotyl segments near to the root of 2-week-old seedlings on the Murashige and Skoog (MS) basal medium supplemented with 10 µM benzylaminopurine (BAP). However, the MS medium with 6 µM BAP and 6 µM gibberellic acid (GA₃) was most suitable for shoot multiplication and further growth. Shoots longer than 2 cm cultured on the half-strength MS medium supplemented with 0.5 µM naphthaleneacetic acid (NAA) produced a maximum number of roots per shoot. Regenerated plantlets could be successfully acclimatized.

Keywords: auxin; cytokinin; gibberellic acid; histological analysis; shoot multiplication and elongation

Subjects: acclimatization, acclimation; auxins; cytokinins; Eucommia ulmoides; gibberellic acid, gibberellins; hypocotyl; in vitro culture, growth control; in vitro culture, regeneration; nutrient medium, Murashige and Skoog (MS)

Received: June 27, 2006; Accepted: May 16, 2007; Published: December 1, 2008Show citation