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Biologia plantarum 52:718-722, 2008 | DOI: 10.1007/s10535-008-0138-9

Specific activation of PR-10 pathogenesis-related genes in apple by an incompatible race of Venturia inaequalis

M. Chevalier1, L. Parisi2, B. Gueye3, C. Campion3, P. Simoneau3, P. Poupard3,*
1 UMR GENHORT 1259, Unité d'Amélioration des Espèces Fruitières et Ornementales, Centre INRA d'Angers, Beaucouzé Cedex, France
2 UMR PAVE 77, Unité de Pathologie Végétale, Centre INRA d'Angers, Beaucouzé Cedex, France
3 UFR Sciences, Université d'Angers, Angers Cedex 01, France

Apple (Malus × domestica Borkh. cv. Golden Delicious) trees were inoculated with fungal pathogens, corresponding either to an incompatible strain of Venturia inaequalis or to non-host pathogens (Venturia pyrina, Alternaria brassicicola) in order to characterize the regulation of PR-10 genes in these different situations in relationship to symptom development. Macro-and microscopic observations of the plant-fungus interactions revealed typical symptoms of resistance with V. inaequalis and symptoms of hypersensitivity (HR) on around 5 % of leaves with V. pyrina. No HR was microscopically observed with A. brassicicola. In the non-host situations, the PR-10 expression in leaves at the transcriptional level was not (A. brassicicola) or very slightly (V. pyrina) activated. This strongly suggests that PR-10 is not a component of the non-host resistance in the interactions studied here. By contrast specific up-regulation of PR-10 was evident after inoculation with V. inaequalis.

Keywords: Alternaria brassicicola; resistance mechanisms; Malus × domestica; Venturia pyrina
Subjects: Alternaria brassiciola; apple; Malus domestica; Venturia inaequalis; Venturia pyrina

Received: October 14, 2006; Accepted: June 12, 2007; Published: December 1, 2008Show citation

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Chevalier, M., Parisi, L., Gueye, B., Campion, C., Simoneau, P., & Poupard, P. (2008). Specific activation of PR-10 pathogenesis-related genes in apple by an incompatible race of Venturia inaequalis. Biologia plantarum52(4), 718-722. doi: 10.1007/s10535-008-0138-9.
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References

  1. Bénaouf, G., Parisi, L.: Genetics of host-pathogen relationships between Venturia inaequalis races 6 and 7 and Malus species.-Phytopathology 90: 236-242, 2000. Go to original source...
  2. Chen, Z.Y., Brown, R.L., Rajasekaran, K., Damann, K.E., Cleveland, T.E.: Identification of a maize kernel pathogenesis-related protein and evidence for its involvement in resistance to Aspergillus flavus infection and aflatoxin production.-Phytopathology 96: 87-95, 2006. Go to original source...
  3. Chevalier, M., Lespinasse, Y., Renaudin, S.: A microscopic study of different classes of symptoms by the Vf gene in apple for resistance to scab (Venturia inaequalis).-Plant Pathol. 40: 249-256, 1991. Go to original source...
  4. Constabel, C.P., Brisson, N.: The defense-related STH-2 gene product of potato shows race-specific accumulation after inoculation with low concentrations of Phytophthora infestans and Potato Virus X.-Plant mol. Biol. 188: 289-295, 1992.
  5. Fristensky, B., Riggleman, R.C., Wagoner, W., Hadwiger, L.A.: Gene expression in susceptible and disease resistant interactions of peas with Fusarium solani pathogens and chitosan.-Physiol. Plant Pathol. 27: 15-28, 1985.
  6. Heath, M.C.: Non-host resistance to plant pathogens: nonspecific defense or the result of specific recognition events?-Physiol. mol. Plant Pathol. 58: 53-54, 2001. Go to original source...
  7. Jwa, N.S., Agrawal, G.K., Rakwal, R., Park, C.H., Agrawal, V.P.: Molecular cloning and characterization of a novel jasmonate inducible pathogenesis-related class 10 protein gene, JIOsPR10, from rice (Oryza sativa L.) seedlings leaves.-Biochem. biophys. Res. Commun. 286: 973-983, 2001. Go to original source...
  8. Kroes, G.M.L., Baayen, R.P., Lange, W.: Histology for root of flax seedlings (Linum usitatissimum) infected by Fusarium oxysporum f. sp. lini.-Eur. J. Plant Pathol. 104: 725-736, 1998. Go to original source...
  9. Liu, J.J., Ekramoddoullah, A.K.M.: The family 10 of plant pathogenesis-related proteins: their structure, regulation, and function in response to biotic and abiotic stresses.-Physiol. mol. Plant Pathol. 68: 3-13, 2006. Go to original source...
  10. Lo, S.C.C., Hipskind, J.D., Nicholson, R.L.: cDNA cloning of a sorghum pathogenesis-related proteins (PR-10) and differential expression of defense-related genes following inoculation with Cochliobolus heterostrophus or Colletotrichum sublineolum.-Mol. Plant Microbe Interact. 12: 479-489, 1999. Go to original source...
  11. Mould, M.J.R., Xu, T., Barbara, M., Iscove, N.N., Heath, M.C.: cDNAs generated from individual epidermal cells reveal that differential gene expression predicting subsequent resistance or susceptibility to rust fungal infection occurs prior to the fungus entering the cell lumen.-Mol. Plant Microbe Interact. 16: 835-845, 2003. Go to original source...
  12. Mysore, K.S., Ryu, C.M.: Nonhost resistance: how much do we know?-Trends Plant Sci. 9: 97-104, 2004. Go to original source...
  13. Parisi, L., Lespinasse, Y., Guillaumès, J., Krüger, J.: A new race of Venturia inaequalis virulent to apples with resistance due to the Vf gene.-Phytopathology 83: 533-537, 1993. Go to original source...
  14. Pinto, M.P., Ribeiro, A., Regalado, A.P., Rodrigues-Pousada, C., Ricardo, C.P.P.: Expression of Lupinus albus PR-10 proteins during root and leaf development.-Biol. Plant. 49: 187-193, 2005. Go to original source...
  15. Poupard, P., Parisi, L., Campion, C., Ziadi, S., Simoneau, P.: A wound-and ethephon-inducible PR-10 gene subclass from apple is differentially expressed during infection with a compatible and an incompatible race of Venturia inaequalis.-Physiol. mol. Plant Pathol. 62: 3-12, 2003. Go to original source...
  16. Pühringer, H., Moll, D., Hoffmann-Sommergruber, K., Watillon, B., Katinger, H., Laimer da Câmara Machado, M.: The promoter of an apple Ypr10 gene, encoding the major allergen Mal d 1, is stress-and pathogen-inducible.-Plant Sci. 152: 35-50, 2000. Go to original source...
  17. Robert, N., Ferran, J., Breda, C., Coutos-Thévenot, P., Boulay, M., Buffard, D., Esnault, R.: Molecular characterization of the incompatible interaction of Vitis vinifera leaves with Pseudomonas syringae pv. pisi: expression of genes coding for stilbene synthase and class 10 PR protein.-Eur. J. Plant Pathol. 107: 249-261, 2001. Go to original source...
  18. Roux, C., Lemarquand, A., Orain, G., Campion, C., Simoneau, P., Poupard, P.: Effects of the plant growth regulator prohexadione-calcium and the SAR-inducer acibenzolar-S-methyl on the quality of apples at harvest.-J. hort. Sci. Biotechnol. 81: 139-145, 2006. Go to original source...
  19. Steiner-Lange, S., Fischer, A., Boettcher, A., Rouhara, I., Liedgens, H., Schmelze, E., Knogge, W.: Differential defence reactions in leaf tissues of barley in response to infection by Rhynchosporium secalis and to treatment with a fungal avirulence gene product.-Mol. Plant Microbe Interact. 16: 893-902, 2003. Go to original source...
  20. Swoboda, I., Scheiner, O., Heberle-Bors, E., Vicente, O.: cDNa cloning and characterization of three genes, in the Bet v 1 gene family, that encode pathogenesis-related proteins.-Plant Cell Environ. 18: 865-874, 1995. Go to original source...
  21. Thordal-Christensen, H.: Fresh insights into processes of nonhost resistance.-Curr. Opin. Plant Biol. 6: 351-357, 2003. Go to original source...
  22. Truesdell, G.M., Dickman, B.: Isolation of pathogen/stress-inducible cDNAs from alfalfa by mRNA differential display.-Plant mol. Biol. 33: 737-743, 1997. Go to original source...
  23. Van Loon, L.C., Pierpoint, W.S., Boller, T., Conejero, V.: Recommendation for naming plant pathogenesis-related proteins.-Plant mol. Biol. Rep. 12: 245-264, 1994. Go to original source...
  24. Winner, J., Jung, C.K., Shackel, I., Williams, P.M.: Development and validation of real-time quantitative reverse transcriptase-polymerase chain reaction for monitoring gene expression in cardiac myocytes in vitro.-Anal. Biochem. 270: 41-49, 1999. Go to original source...
  25. Ziadi, S., Poupard, P., Brisset, M.N., Paulin, J.P., Simoneau, P.: Characterization in apple leaves of two subclasses of PR-10 transcripts inducible by acibenzolar-S-methyl, a functional analogue of salicylic acid.-Physiol. mol. Plant Pathol. 59: 33-43, 2001. Go to original source...