Biologia plantarum 54:357-360, 2010 | DOI: 10.1007/s10535-010-0063-6

Indirect regeneration of Withania somnifera and comparative analysis of withanolides in in vitro and greenhouse grown plants

Y. H. Dewir1,*, D. Chakrabarty2, S. -H. Lee3, E. -J. Hahn3, K. -Y. Paek3
1 Department of Horticulture, Faculty of Agriculture, Kafrelsheikh University, Kafr El-Sheikh, Egypt
2 Floriculture Section, National Botanical Research Institute, Lucknow, India
3 Research Center for The Development of Advanced Horticultural Technology, Chungbuk National University, Cheong-Ju, Korea

The present study reports an efficient protocol for indirect shoot organogenesis and plantlets regeneration of Withania somnifera (L.) Dunal. Leaf explants were cultured on Murashige and Skoog (MS) medium supplemented with different concentrations and combinations of 6-benzylaminopurine (BAP) and indole-3-acetic acid (IAA). The highest callus induction rate (89.5 %) and shoot regeneration rate (92 %) were obtained when 2 mg dm-3 BAP was combined with 0.5 mg dm-3 IAA. Three major withanolides (withaferine A, 12-deoxywithastramonolide and withanolide A) were investigated in different plant organs from in vitro and greenhouse grown plants. Leaves contained higher contents of withanolides and phenolics than roots or stems, whereas roots contained the highest contents of flavonoids and polysacharides. In vitro grown plants contained greater contents of phenolics, flavonoids and polysaccharides while lower contents of withanolides than greenhouse grown plants.

Keywords: DPPH; flavonoid; phenolics; polysaccharides; shoot organogenesis; withaferine
Subjects: auxins; cytokinins; ex vitro transfer; in vitro culture, callus; in vitro culture, rooting; Whitania somnifera; whitanolides

Received: July 15, 2008; Accepted: January 20, 2009; Published: June 1, 2010Show citation

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Dewir, Y.H., Chakrabarty, D., Lee, S.-H., Hahn, E.-J., & Paek, K.-Y. (2010). Indirect regeneration of Withania somnifera and comparative analysis of withanolides in in vitro and greenhouse grown plants. Biologia plantarum, 54(2), 357-360. doi: 10.1007/s10535-010-0063-6.
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