Biologia plantarum 2013, 57:174-178 | DOI: 10.1007/s10535-012-0252-6

The role of cytokinins during micropropagation of wych elm

J. Malá1, P. Máchová1, H. Cvrčková1, M. Karady2, O. Novák2, J. Mikulík2, J. Dostál1, M. Strnad2,3, K. Doležal2,3,*
1 Forestry and Game Management Research Institute, Jíloviště, Czech Republic
2 Laboratory of Growth Regulators, Palacký University and Institute of Experimental Botany AS CR, Olomouc-Holice, Czech Republic
3 Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, Olomouc, Czech Republic

We have compared the influence of two aromatic cytokinin derivatives, N 6-benzyladenine (BA) and meta-topolin (mT), on the in vitro multiplication and senescence of wych elm (Ulmus glabra Huds.). After 3 months of cultivation, the micropropagation rate was higher (approx. six times more shoots developed) on Murashige and Skoog (MS) medium supplemented with mT than on MS supplemented with BA. Quantification of 50 endogenous cytokinins, using a recently developed UPLC-ESI(+)-MS/MS method, showed significant differences in the cytokinin metabolites (especially different glucosides) in explants cultivated on media supplemented with BA or mT. Differences in efficiency of photosystem II was also detected.

Keywords: benzylaminopurine; chlorophyll fluorescence; meta-topolin; organogenesis; rooting; senescence
Subjects: in vitro culture; cytokinin; chlorophyll fluorescence; meta-topolin; senescence; wych elm
Species: Ulmus glabra

Received: July 29, 2011; Accepted: April 5, 2012; Published: March 1, 2013Show citation

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Malá, J., Máchová, P., Cvrčková, H., Karady, M., Novák, O., Mikulík, J., ... Doležal, K. (2013). The role of cytokinins during micropropagation of wych elm. Biologia plantarum, 57(1), 174-178. doi: 10.1007/s10535-012-0252-6.
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