Biologia plantarum 58:667-675, 2014 | DOI: 10.1007/s10535-014-0451-4

COR15B expression is affected by chloroplast functionality and its role in response to salt stress in Arabidopsis thaliana

D. Liu1,*, L. Hou1, W. C. Li1, J. F. Cheng1, Y. Q. Fu1
1 College of Agronomy, Jiangxi Agricultural University, Nanchang, P.R. China

Cold-responsive (COR) genes participate in the response of plants to low-temperature stress. In this study, we isolated and characterized a cold-responsive and light-inducible gene COR15B from Arabidopsis thaliana. Chloroplast damage caused by mutations (albino mutants seca1, secy1, and tic20) or by a norflurazon (NF) treatment resulted in a reduction of COR15B transcription. A semi-quantitative RT-PCR analysis shows that COR15B was induced by the salt stress in an abscisic acid-dependent manner. An over-expression of COR15B in Arabidopsis resulted in transgenic lines more sensitive to the NaCl treatment than the wild type. However, COR15B knockdown did not significantly affect the sensitivity of the cor15b mutant to the salt stress. Furthermore, we found that the expression of COR15A, a homologous gene of COR15B, was significantly elevated in cor15b mutant plants. All these results suggest that plants acquire the ability to fully express COR15B only after development of functional chloroplasts. The expressional reprogramming and functional backup may exist between COR15 homologues in Arabidopsis.

Keywords: abscisic acid; expressional reprogramming; low temperature; mutants; NaCl; norflurazon
Subjects: gene expression; chloroplast; salt stress; abscisic acid; temperature - low; mutants; norflurazon; germination; greening
Species: Arabidopsis thaliana

Received: January 3, 2014; Revised: June 3, 2014; Accepted: June 11, 2014; Published: December 1, 2014Show citation

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Liu, D., Hou, L., Li, W.C., Cheng, J.F., & Fu, Y.Q. (2014). COR15B expression is affected by chloroplast functionality and its role in response to salt stress in Arabidopsis thaliana. Biologia plantarum, 58(4), 667-675. doi: 10.1007/s10535-014-0451-4.
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