Biologia plantarum 49:423-426, 2005 | DOI: 10.1007/s10535-005-0020-y

Plant regeneration in vitro directly from cotyledon and hypocotyl explants of Perilla frutescens and their morphological aspects

T. Zhang1,2, X. Y. Wang1,*, Z. Y. Cao1,3
1 The Institute of Cell Biology, Lanzhou University, Lanzhou, P.R. China
2 Gansu Academy of Agricultural Science, Lanzhou, P.R. China
3 School of Life Science and Technology, Gansu Agricultural University, Lanzhou, P.R. China

A rapid plantlet regeneration system for Perilla frutescens was established from cotyledon and hypocotyl explants. A maximum of 91.06 % cotyledon and 76.4 % hypocotyl explants could directly produce shoots (3.09 ± 0.18 shoots per explants) on Murashige and Skoog (MS) medium. The optimum hormone combinations were 4.44 μM 6-benzylaminopurine (BA) for cotyledon and 2.22 μM BA + 2.85 μM indole-3-acetic acid (IAA) for hypocotyls. Rooting was induced on half-strength hormone-free MS medium. After transplantation to soil, approximate 80 % of the regenerated plantlets could survive, flower and fruit. Moreover, some morphological abnormalities were found among the regenerated plants.

Keywords: growth regulators; direct organogenesis; morphological abnormalities
Subjects: auxins; cotyledons; cytokinins; hypocotyl; in vitro culture, regeneration, organogenesis, morphogenesis; medicinal plants; nutrient medium MS (Murashige and Skoog); Perilla frutescens

Received: November 12, 2003; Accepted: November 4, 2004; Published: September 1, 2005Show citation

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Zhang, T., Wang, X.Y., & Cao, Z.Y. (2005). Plant regeneration in vitro directly from cotyledon and hypocotyl explants of Perilla frutescens and their morphological aspects. Biologia plantarum, 49(3), 423-426. doi: 10.1007/s10535-005-0020-y.
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