Biologia plantarum 2014, 58:385-390 | DOI: 10.1007/s10535-014-0399-4

Identification of a C2H2-type zinc finger gene family from Eucalyptus grandis and its response to various abiotic stresses

S. Wang1, X. L. Wei1, L. J. Cheng1,*, Z. K. Tong1
1 The Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang Agriculture and Forestry University, Lin'an, P.R. China

C2H2-type zinc finger proteins belong to a major family of transcription factors that play a crucial role in plant stress responses. In this study, seven C2H2-type zinc finger genes (EgrZFP1-7) in Eucalyptus grandis were cloned using the RACE PCR method. EgrZFP1-7 proteins included a QALGGH motif, two zinc finger domains, and an EAR motif. They belong to a Q-type C2H2 zinc finger protein family and are classified into the subset C1. EgrZFP4 and EgrZFP6 had a higher transcription in roots than in leaves and stems, whereas the expression of the other genes did not differ in various plant tissues. The expression of EgrZFP genes in seedlings was induced by low temperatures. Time course experiments at temperatures lower than 4 °C revealed oscillating expression of EgrZFP1-6 during the cold treatment. However, EgrZFP7 showed a phasic expression pattern at the same conditions. The expression of EgrZFP1-6 was found to be enhanced by 200 mM NaCl, whereas the expression of EgrZFP7 was inhibited.

Keywords: gene expression; low temperature; NaCl; RACE PCR; transcription factors
Subjects: C2H2-type zinc finger protein; transcription factor; gene expression; temperature - low; salinity; amino acid sequence; phylogenetic analysis; eucalypt
Species: Eucalyptus grandis

Received: March 13, 2013; Revised: August 28, 2013; Accepted: September 6, 2013; Published: June 1, 2014Show citation

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Wang, S., Wei, X.L., Cheng, L.J., & Tong, Z.K. (2014). Identification of a C2H2-type zinc finger gene family from Eucalyptus grandis and its response to various abiotic stresses. Biologia plantarum, 58(2), 385-390. doi: 10.1007/s10535-014-0399-4.
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