Biologia plantarum 61:106-114, 2017 | DOI: 10.1007/s10535-016-0644-0

Quantitative proteomic analysis of upland cotton stem terminal buds reveals phytohormone-related pathways associated with dwarfism

X. Tu1,2, J. Li2, Q. Wang3,*, A. Liu2,*
1 College of Orient Science and Technology, Hunan Agricultural University, Changsha, P.R. China
2 College of Agronomy, Hunan Agricultural University, Changsha, P.R. China
3 College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, P.R. China

Identifying important regulative elements and pathways related to dwarfism in cotton is a major challenge in cotton breeding. Isobaric tags for relative and absolute quantitation-based proteomics of stem terminal buds from upland cotton (Gossypium hirsutum L.) dwarf line LA-1 and high near-isogenic line LH-1 was performed. Moreover, transcriptional expression of differentially-expressed proteins (DEPs) belonging to phytohormone signal transduction, hormone biosynthesis, and ubiquitin system were analysed using quantitative real-time polymerase chain reaction. A total of 4 849 proteins were identified from LA-1 and LH-1, 697 of which showed differential accumulations. Most of the DEPs have catalytic, binding, and transporter activity and are involved in metabolism- and protein processing-related pathways. In particular, seven DEPs, including two gibberellin (GA) receptors, three cytokinin (CK) receptors, CKoxidase, and CK-N-glucosyltransferase were up-regulated in LA-1, and GA20-oxidase was down-regulated in LH-1. Our results suggest that the DELLA-independent GAsignalling pathway is the primary cause of dwarfism in LA-1 and indicate that CKresponse element 1-2, GA-insensitive dwarf, CKoxidase, and GA3-β-dioxygenase are potential indicators of dwarf cotton. The profiling of DEPs may offer a valuable resource for cotton breeding.

Keywords: auxins; cytokinins; gibberellins; Gossypium hirsutum; iTRAQ; near-isogenic line
Subjects: proteomics; dwarfism; auxin; cytokinin; gibberellin; iTRAQ; near-isogenic line; growth; gene expression; cotton
Species: Gossypium hirsutum

Received: June 15, 2015; Revised: January 31, 2016; Accepted: March 14, 2016; Published: January 1, 2017Show citation

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Tu, X., Li, J., Wang, Q., & Liu, A. (2017). Quantitative proteomic analysis of upland cotton stem terminal buds reveals phytohormone-related pathways associated with dwarfism. Biologia plantarum, 61(1), 106-114. doi: 10.1007/s10535-016-0644-0.
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