Biologia Plantarum 63: 97-103, 2019 | DOI: 10.32615/bp.2019.012

Genome-wide identification of circular RNAs in tomato seeds in response to high temperature

R. Zhou1,2, X.Q. Yu3, L.P. Xu4, Y.L. Wang1,2, L.P. Zhao1,2, T.M. Zhao1,2,*, W.G. Yu1,2,*
1 Institute of Vegetable Crop, Jiangsu Province Academy of Agricultural Sciences, Jiangsu, 210014 Nanjing, P.R. China
2 Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Jiangsu, 210014 Nanjing, P.R. China
3 College of Horticulture, Nanjing Agricultural University, Jiangsu, 210095 Nanjing, P.R .China
4 Institute of Vegetable Research in Xining, Qinghai, 810000 Xining, P.R. China

Circular RNAs (circRNAs), an emerging class of non-coding RNAs, are abundant in eukaryotic transcriptomes. Seed germination is one of the most important stages in the entire life cycle of plants that can be slowed down or totally restrained by high temperature. Our aim is to identify heat-responsive circRNAs and explore the potential function of circRNAs in tomato seeds at high temperature. Following high-throughput sequencing, 4 164 circRNAs were identified, and 980 circRNAs were shared in the control and high-temperature libraries. Among the 748 circRNAs with high expressions, 73 circRNAs were significantly up-/down- regulated in tomato seeds germinated at high temperature compared to the control. The parental genes of circRNAs existing in seeds only at high temperature were mainly involved in metabolic processes, cellular processes, catalytic activities, and binding based on Gene Ontology analysis. The results suggested that circRNAs were widespread in tomato and were generated from different chromosomes and diverse genomic regions. Some circRNAs in tomato seeds responded to high temperature during germination. This study provides the first genome-wide profile of circRNAs in response to high temperature during tomato seed germination and lays a foundation for studying the potential biological functions of circRNAs responding to heat stress.

Keywords: chromosome distribution, heat stress, non-coding RNAs, seed germination, Solanum lycopersicum

Accepted: November 13, 2018; Prepublished online: November 14, 2018; Published online: January 19, 2019Show citation

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Zhou, R., Yu, X.Q., Xu, L.P., Wang, Y.L., Zhao, L.P., Zhao, T.M., & Yu, W.G. (2019). Genome-wide identification of circular RNAs in tomato seeds in response to high temperature. Biologia plantarum, 63, 97-103. doi: 10.32615/bp.2019.012.
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