Biologia plantarum 2011, 55:213-224 | DOI: 10.1007/s10535-011-0032-8

Genetic transformation of barley: limiting factors

Š. Vyroubalová1, M. Šmehilová1, P. Galuszka1, L. Ohnoutková2,*
1 Department of Biochemistry, Palacký University in Olomouc, Olomouc, Czech Republic
2 Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Olomouc, Czech Republic

This review summarizes main difficulties involved in barley (Hordeum vulgare L.) transformation. The most commonly used procedures for genetic transformation in barley are Agrobacterium tumefaciens and particle bombardment mediated methods. While different barley cultivars are used for genetic engineering with varying sensitivity, recent improvements in regeneration and transformation techniques are described and summarized. Furthermore, some of the transformation complicating factors, in particular somaclonal variation and transgene insertion sites, are discussed in more detail.

Keywords: Agrobacterium; albinism; Hordeum; particle bombardment; somaclonal variation; transgene insertion site

Received: November 23, 2009; Accepted: May 5, 2010; Published: June 1, 2011Show citation

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Vyroubalová, Š., Šmehilová, M., Galuszka, P., & Ohnoutková, L. (2011). Genetic transformation of barley: limiting factors. Biologia plantarum, 55(2), 213-224. doi: 10.1007/s10535-011-0032-8.
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