Biologia plantarum 54:272-278, 2010 | DOI: 10.1007/s10535-010-0047-6

Assessment of genetic diversity and relationships among Coix lacryma-jobi accessions using microsatellite markers

K. -H. Ma1,2, K. -H. Kim2, A. Dixit1,3, I. -M. Chung2, J. -G. Gwag1, T. -S. Kim1, Y. -J. Park4,*
1 National Agrobiodiversity Center, National Academy of Agricultural Science, Suwon, Republic of Korea
2 College of Life and Environmental Sciences, Konkuk University, Seoul, Republic of Korea
3 Basmati Export Development Foundation, Meerut, India
4 Department of Plant Resources, College of Industrial Sciences, Kongju National University, Yesan, Republic of Korea

The present study describes the assessment of genetic diversity and relationships among 79 Job's tears (Coix lacrymajobi L.) accessions collected from China and Korea using 17 microsatellite markers. A total of 57 alleles were detected with an average of 3.4 alleles per locus. A high frequency of rare alleles (36.3 %) was observed within the collection. Values for observed (HO), expected heterozygosity (HE) and Shannon's information index (I) within the analysis ranged from 0.00 (GBssrJT183) to 0.81 (GBssrJT130), from 0.01 (GBssrJT170) to 0.65 (GBssrJT130) and from 0.034 (GBssrJt170) to 1.13 (GBssrJT130), respectively. The locus GBJT130 was the most informative marker with the highest values for observed and effective alleles as well as for HO, HE and I. Based on the UPGMA algorithm, the majority of the Chinese accessions grouped in one cluster, whereas all the Korean accessions grouped together in a separate cluster, indicating that Chinese accessions are genetically quite distinct from Korean accessions. No relation between genetic relatedness among Job's tears accessions and their place of collection was observed. Chinese accessions exhibited greater within population polymorphism (P = 95 %, HE = 0.30, I = 0.52) than the accessions from Korea (P = 68 %, HE = 0.13, I =0.24), indicating their potentiality as a reservoir of novel alleles for crop improvement. However, in general the low diversity within each population indicates a narrow genetic base within our collection.

Keywords: heterozygosity; Job's tears; polymorphism; simple sequence repeats
Subjects: Coix lacryma-jobi; genetic diversity; heterozygosity; microsatellite markers; simple sequence repeats (SSR); UPGMA dengrogram

Received: April 17, 2007; Accepted: December 20, 2008; Published: June 1, 2010Show citation

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Ma, K.-H., Kim, K.-H., Dixit, A., Chung, I.-M., Gwag, J.-G., Kim, T.-S., & Park, Y.-J. (2010). Assessment of genetic diversity and relationships among Coix lacryma-jobi accessions using microsatellite markers. Biologia plantarum, 54(2), 272-278. doi: 10.1007/s10535-010-0047-6.
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